TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and placenta during pregnancy.
TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and placenta during pregnancy.
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DOI：
10.1186/1477-7827-10-66
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发表时间：
2012-08-29
期刊：
影响因子：
--
通讯作者：
Boos A
中科院分区：
文献类型：
--
作者：
Sprekeler N;Kowalewski MP;Boos A
瞬时受体电位通道6（TRPV 6）和钙结合蛋白-D9 k（CaBP-9 k）参与了包括胎盘和子宫在内的许多组织中的主动钙（Ca 2+）转运机制，表明在妊娠的建立和维持中起作用。此外，TRPV 6和CaBP-9 k似乎支持母胎Ca 2+转运，这对胎儿Ca 2+稳态、骨生长和发育至关重要。然而，尚不清楚这些蛋白质是否也参与了与奶牛分娩相关的病理学，如胎膜残留（RFM）。本研究的目的是建立子宫和胎盘TRPV 6和CaBP-9 k mRNA和蛋白质在妊娠和产后的表达谱，有和没有胎膜释放的奶牛。收集了27头不同妊娠期奶牛的子宫和胎盘组织以及有无RFM奶牛的胎盘组织。采用实时荧光定量PCR、免疫组化和Western blot检测TRPV 6和CaBP-9 k蛋白和mRNA的表达。在子宫内膜中，最高TRPV 6和CaBP-9 k的表达被发现在妊娠的最后三个月，特别是在腺上皮中的蛋白质的增加。在胎盘中，TRPV 6 mRNA的逐渐增加是可检测到的分娩，而蛋白质表达没有显着变化。胎盘组织CaBP-9 k的表达在整个妊娠期间没有显著变化，但免疫组化显示母体隐窝上皮的染色强度增加。免疫组织化学，更强的胎盘CaBP-9 k信号被认为是在动物RFM与未受干扰的胎膜释放相比，而蛋白质水平，通过蛋白质印迹分析测量没有显着变化。本研究的结果表明，TRPV 6和CaBP-9 k的动态表达在怀孕期间的牛子宫内膜和胎盘，这表明这些蛋白质在怀孕期间的钙代谢的功能作用。时间和空间表达模式表明，TRPV 6和CaBP-9 k可能参与母胎Ca 2+转运，主要是通过胎盘间转运，这两种蛋白质可能参与对胎儿和胎盘发育至关重要的生理过程。然而，TRPV 6和CaBP-9 k似乎都不是胎膜滞留的原因。
Transient receptor potential channel type 6 (TRPV6) and Calbindin-D9k (CaBP-9k) are involved in the active calcium (Ca2+) transport mechanism in many tissues including placenta and uterus, suggesting a role in the establishment and maintenance of pregnancy. Moreover, TRPV6 and CaBP-9k seem to support the materno-fetal Ca2+ transport that is crucial for fetal Ca2+ homeostasis, bone growth and development. However, it is unknown if these proteins are also involved in the aetiology of pathologies associated with parturition in cows, such as retained fetal membranes (RFM). The aim of the current study was to create an expression profile of uterine and placentomal TRPV6 and CaBP-9k mRNAs and proteins during pregnancy and postpartum in cows with and without fetal membrane release. Uteri and placentomes of 27 cows in different stages of pregnancy and placentomes of cows with and without RFM were collected. Protein and mRNA expression of TRPV6 and CaBP-9k was investigated by real-time PCR, immunohistochemistry and Western blot. In the uterine endometrium, highest TRPV6 and CaBP-9k expression was found in the last trimester of pregnancy, with a particular increase of protein in the glandular epithelium. In the placentomes, a gradual increase in TRPV6 mRNA was detectable towards parturition, while protein expression did not change significantly. Placentomal CaBP-9k expression did not change significantly throughout pregnancy but immunohistochemistry revealed an increase in staining intensity in the maternal crypt epithelium. Immunohistochemical, stronger placental CaBP-9k signals were seen in animals with RFM compared to animals with an undisturbed fetal membrane release, while protein levels, measured by Western blot analyses did not change significantly. The results of the present study demonstrate a dynamic expression of TRPV6 and CaBP-9k during pregnancy in the bovine uterine endometrium and placentomes, suggesting a functional role for these proteins in Ca2+ metabolism during pregnancy. The temporal and spatial expression patterns indicate that TRPV6 and CaBP-9k may be involved in materno-fetal Ca2+ transport, mainly through an interplacentomal transport, and that both proteins may participate in physiological processes that are crucial for fetal and placental development. However, neither TRPV6 nor CaBP-9k seem to be causative in the retention of fetal membranes.
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影响因子：
4.1
作者：
An, BS;Choi, KC;Jeung, EB
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Jeung, EB
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3.6
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2.6
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影响因子：
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4.4
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