No evidence for trans-generational immune priming in Drosophila melanogaster.
No evidence for trans-generational immune priming in Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0288342
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Lazzaro, Brian P.
中科院分区：
文献类型：
--
作者：
Radhika, R.;Lazzaro, Brian P.
大多数生物体都处于不断和反复暴露于病原体的环境中，导致自然选择以更有效的方式抵抗感染。这可能包括基于记忆的免疫进化，以增加对世代内和世代间反复遇到的病原体的保护。在缺乏脊椎动物抗体介导的获得性免疫特性的非脊椎动物中，有不同的证据表明存在代内启动和跨代启动。在这项工作中，我们测试了在母体用10种不同的细菌病原体攻击后，果蝇成年后代的跨代免疫启动。我们关注的是果蝇的自然条件致病菌，其毒力范围从10%到100%不等。我们通过脓毒性损伤感染母鼠，并测试其成年后代对感染的抵抗力增强，以抑制细菌增殖和存活感染的能力来衡量。我们根据测试的每种细菌将母亲分为四类：感染后存活的、感染后死亡的、无菌损伤控制的和未损伤控制的。我们没有发现任何类型的母亲对任何细菌的反应有跨代启动的证据。
Most organisms are under constant and repeated exposure to pathogens, leading to perpetual natural selection for more effective ways to fight-off infections. This could include the evolution of memory-based immunity to increase protection from repeatedly-encountered pathogens both within and across generations. There is mixed evidence for intra- and trans-generational priming in non-vertebrates, which lack the antibody-mediated acquired immunity characteristic of vertebrates. In this work, we tested for trans-generational immune priming in adult offspring of the fruit fly, Drosophila melanogaster, after maternal challenge with 10 different bacterial pathogens. We focused on natural opportunistic pathogens of Drosophila spanning a range of virulence from 10% to 100% host mortality. We infected mothers via septic injury and tested for enhanced resistance to infection in their adult offspring, measured as the ability to suppress bacterial proliferation and survive infection. We categorized the mothers into four classes for each bacterium tested: those that survived infection, those that succumbed to infection, sterile-injury controls, and uninjured controls. We found no evidence for trans-generational priming by any class of mother in response to any of the bacteria.
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