Multi-kingdom characterization of the core equine fecal microbiota based on multiple equine (sub)species.
Multi-kingdom characterization of the core equine fecal microbiota based on multiple equine (sub)species.
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DOI：
10.1186/s42523-020-0023-1
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发表时间：
2020-02-12
影响因子：
4.7
通讯作者：
Smidt H
中科院分区：
文献类型：
--
作者：
Edwards JE;Shetty SA;van den Berg P;Burden F;van Doorn DA;Pellikaan WF;Dijkstra J;Smidt H
迄今为止，马肠道微生物学研究主要集中在马和矮种马上，它们仅代表现存的八种马物种之一。尽管驴和骡子几乎占世界上驯养的马的一半，而且驴在降解膳食纤维的能力方面优于马/小马。尽管厌氧真菌是有效的纤维降解生物，其活性被产甲烷古菌增强，但对纤维厌氧真菌和古菌的关注也有限。因此，本研究的目的是将马粪便微生物群的细菌、厌氧真菌和古菌多样性的现有知识扩展到多个马物种。确定了所有马粪便样本（n = 70）共有的核心分类群，并概述了不同马类型（马、驴、马×驴和斑马）的微生物群。马的类型与粪便微生物浓度和群落组成的差异有关。驴通常与其他马科动物最不同，马和斑马没有区别。尽管如此，在所有粪便样品中发现了8个OTU（2070个中）和16个属水平分组（231个中）的共同细菌核心。该细菌核心代表了比先前报道的更大比例的马粪便微生物群，主要是由于检测到属于Kiritimatiellaeota门（以前为Verrucomicrobia亚门5）和螺旋体属的主要核心分类群。大多数核心细菌类群缺乏培养的代表性。在所有动物中均存在嗜热菌和厌氧真菌，然而，尽管有几个类群是普遍和占主导地位的，但没有检测到核心类群。虽然在马类型之间观察到差异，但所有马都存在核心粪便微生物群。该核心主要由少数优势细菌类群组成，其中大多数是新的，缺乏培养的代表性。缺乏代表主要类群的微生物培养物需要得到解决，因为它们的可用性对于获得支撑马后肠生态系统的微生物功能的基本知识至关重要。
Equine gut microbiology studies to date have primarily focused on horses and ponies, which represent only one of the eight extant equine species. This is despite asses and mules comprising almost half of the world’s domesticated equines, and donkeys being superior to horses/ponies in their ability to degrade dietary fiber. Limited attention has also been given to commensal anaerobic fungi and archaea even though anaerobic fungi are potent fiber degrading organisms, the activity of which is enhanced by methanogenic archaea. Therefore, the objective of this study was to broaden the current knowledge of bacterial, anaerobic fungal and archaeal diversity of the equine fecal microbiota to multiple species of equines. Core taxa shared by all the equine fecal samples (n = 70) were determined and an overview given of the microbiota across different equine types (horse, donkey, horse × donkey and zebra). Equine type was associated with differences in both fecal microbial concentrations and community composition. Donkey was generally most distinct from the other equine types, with horse and zebra not differing. Despite this, a common bacterial core of eight OTUs (out of 2070) and 16 genus level groupings (out of 231) was found in all the fecal samples. This bacterial core represented a much larger proportion of the equine fecal microbiota than previously reported, primarily due to the detection of predominant core taxa belonging to the phyla Kiritimatiellaeota (formerly Verrucomicrobia subdivision 5) and Spirochaetes. The majority of the core bacterial taxa lack cultured representation. Archaea and anaerobic fungi were present in all animals, however, no core taxon was detected for either despite several taxa being prevalent and predominant. Whilst differences were observed between equine types, a core fecal microbiota existed across all the equines. This core was composed primarily of a few predominant bacterial taxa, the majority of which are novel and lack cultured representation. The lack of microbial cultures representing the predominant taxa needs to be addressed, as their availability is essential to gain fundamental knowledge of the microbial functions that underpin the equine hindgut ecosystem.
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影响因子：
2.6
作者：
Costa MC;Stämpfli HR;Arroyo LG;Allen-Vercoe E;Gomes RG;Weese JS
通讯作者：
Weese JS
影响因子：
3.7
作者：
Dougal K;de la Fuente G;Harris PA;Girdwood SE;Pinloche E;Geor RJ;Nielsen BD;Schott HC 2nd;Elzinga S;Newbold CJ
通讯作者：
Newbold CJ
DOI：
10.1017/s1357729800013953
发表时间：
1995-10-01
期刊：
ANIMAL SCIENCE
影响因子：
--
作者：
CUDDEFORD, D;PEARSON, RA;MUIRHEAD, RH
通讯作者：
MUIRHEAD, RH
影响因子：
2.2
作者：
Costa, M. C.;Silva, G.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
2.8
作者：
CWYK, WM;CANALEPAROLA, E
通讯作者：
CANALEPAROLA, E