Dielectrophoretic stretching of DNA tethered to a fiber tip.
Dielectrophoretic stretching of DNA tethered to a fiber tip.
复制标题
DOI：
10.1088/0957-4484/26/12/125501
复制
发表时间：
2015-03-27
期刊：
影响因子：
3.5
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Hyun C;Kaur H;McNabb DS;Li J
在这项工作中，我们研究了固定在光纤尖端上的λ噬菌体DNA分子在交流电场作用下的拉伸。我们在一个小室中设计了一个双电极拉伸系统：一个是镀金光纤尖端电极，另一个是镀金平板电极。通过施加介电泳力，固定在末端的λDNA分子被拉伸，并通过荧光显微镜监测拉伸过程。通过改变电极形状、电极间距、交流频率和溶液电导率，优化了DNA在三维空间中的拉伸。通过观察石英音叉的振动幅度变化，测量了焦耳加热和介质电泳力对溶液中束缚的DNA分子的影响。这项工作展示了一种在探针尖端操作和表征固定化DNADNA分子的方法，用于进一步研究单λ分子。
In this work, we studied the stretching of λ phage DNA molecules immobilized on an optical fiber tip attached to a force sensitive tuning fork under AC electric fields. We designed a two electrodes stretching system in a small chamber: one is a gold-coated optical fiber tip electrode, and the other is a gold-coated flat electrode. By applying a dielectrophoretic force, the immobilized λ DNA molecules on the tip are stretched and the stretching process is monitored by a fluorescent microscope. The DNA stretching in three-dimensional space is optimized by varying electrode shape, electrode gap distance, AC frequency, and solution conductivity. By observing the vibrational amplitude change of a quartz tuning fork, we measured the effects due to Joule heating and the dielectrophoretic force on the tethered DNA molecules in solution. This work demonstrates a method to manipulate and characterize immobilized λ DNA molecules on a probe tip for further study of single DNA molecules.
登录
查看更多内容
影响因子：
17.1
作者：
Hyun, Changbae;Kaur, Harpreet;Rollings, Ryan;Xiao, Min;Li, Jiali
通讯作者：
Li, Jiali
影响因子：
3.5
作者：
Kim, K;Seo, Y;Jhe, W
通讯作者：
Jhe, W
影响因子：
19.6
作者：
Keyser, Ulrich F.;Koeleman, Bernard N.;Dekker, Cees
通讯作者：
Dekker, Cees
影响因子：
3.9
作者：
Bakewell, DJ;Morgan, H
通讯作者：
Morgan, H
影响因子：
3.9
作者：
Lynch, BP;Hilton, AM;Simpson, GJ
通讯作者：
Simpson, GJ