A P-Loop NTPase Regulates Quiescent Center Cell Division and Distal Stem Cell Identity through the Regulation of ROS Homeostasis in Arabidopsis Root.
A P-Loop NTPase Regulates Quiescent Center Cell Division and Distal Stem Cell Identity through the Regulation of ROS Homeostasis in Arabidopsis Root.
复制标题
P 环 NTPase 通过调节拟南芥根中 ROS 稳态来调节静止中心细胞分裂和远端干细胞身份
DOI：
10.1371/journal.pgen.1006175
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发表时间：
2016-09
期刊：
影响因子：
4.5
通讯作者：
Ding Z
中科院分区：
文献类型：
--
作者：
Yu Q;Tian H;Yue K;Liu J;Zhang B;Li X;Ding Z
活性氧是细胞分裂和分化的重要调节因子。APP 1编码的拟南芥P-环NTR通过控制局部活性氧稳态影响根干细胞生态位特性。APP 1的破坏伴随着ROS水平的降低、静止中心（QC）中细胞分裂速率的升高和根远端干细胞（DSC）分化的促进。通过暴露于甲基紫精（MV）和用过氧化氢（H2 O2）处理在app 1突变体中诱导的较高水平的ROS拯救了突变体表型，这意味着QC中细胞分裂速率的增加和根DSC分化的增强都可以归因于低水平的ROS。APP 1在根尖分生组织细胞线粒体中表达，其产物与ATP水解酶活性相关。在app 1突变体中，决定根远端茎生态位的关键转录因子SCARECROW（SCR）和SHORT ROOT（SHR）在转录和蛋白水平上均显著下调，表明SHR和SCR是APP 1调控的ROS信号传导的重要下游靶点，从而控制根QC和DSCs的身份。
Reactive oxygen species (ROS) are recognized as important regulators of cell division and differentiation. The Arabidopsis thaliana P-loop NTPase encoded by APP1 affects root stem cell niche identity through its control of local ROS homeostasis. The disruption of APP1 is accompanied by a reduction in ROS level, a rise in the rate of cell division in the quiescent center (QC) and the promotion of root distal stem cell (DSC) differentiation. Both the higher level of ROS induced in the app1 mutant by exposure to methyl viologen (MV), and treatment with hydrogen peroxide (H2O2) rescued the mutant phenotype, implying that both the increased rate of cell division in the QC and the enhancement in root DSC differentiation can be attributed to a low level of ROS. APP1 is expressed in the root apical meristem cell mitochondria, and its product is associated with ATP hydrolase activity. The key transcription factors, which are defining root distal stem niche, such as SCARECROW (SCR) and SHORT ROOT (SHR) are both significantly down-regulated at both the transcriptional and protein level in the app1 mutant, indicating that SHR and SCR are important downstream targets of APP1-regulated ROS signaling to control the identity of root QC and DSCs.
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影响因子：
64.5
作者：
Cruz-Ramírez A;Díaz-Triviño S;Blilou I;Grieneisen VA;Sozzani R;Zamioudis C;Miskolczi P;Nieuwland J;Benjamins R;Dhonukshe P;Caballero-Pérez J;Horvath B;Long Y;Mähönen AP;Zhang H;Xu J;Murray JA;Benfey PN;Bako L;Marée AF;Scheres B
通讯作者：
Scheres B
影响因子：
20.3
作者：
Lewandowski, Daniel;Barroca, Vilma;Romeo, Paul-Henri
通讯作者：
Romeo, Paul-Henri
拟南芥伸长复合体亚基 2 表观遗传影响根发育。
影响因子：
6.9
作者：
Jia Y;Tian H;Li H;Yu Q;Wang L;Friml J;Ding Z
通讯作者：
Ding Z
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
9.4
作者：
Dunand, Christophe;Crevecoeur, Michele;Penel, Claude
通讯作者：
Penel, Claude