Microarray Analysis of the Juvenile Hormone Response in Larval Integument of the Silkworm, Bombyx mori.
Microarray Analysis of the Juvenile Hormone Response in Larval Integument of the Silkworm, Bombyx mori.
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DOI：
10.1155/2014/426025
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发表时间：
2014
影响因子：
2.9
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Cheng D;Peng J;Meng M;Wei L;Kang L;Qian W;Xia Q
昆虫幼体激素（JH）与20-羟基蜕皮激素（20E）协同调节幼虫生长和蜕皮。然而，这种合作控制在幼虫阶段是如何实现的，人们知之甚少。本研究采用JHA类似物（JHA）甲基戊二烯诱导家蚕超幼，并利用微阵列技术研究JHA对家蚕被膜mRNA表达的影响。我们发现，施用JHA显著增加了参与基本代谢过程和蛋白质加工的大多数基因的表达水平，并降低了与被膜氧化磷酸化相关的基因的表达。与胰岛素/胰岛素样生长因子信号通路（IIS）和20E信号通路相关的几个关键基因也在JHA应用后上调。综上所述，我们认为JH可能通过调节多种代谢途径介导营养依赖性IIS通路，并进一步调节20E信号通路。
Juvenile hormone (JH) coordinates with 20-hydroxyecdysone (20E) to regulate larval growth and molting in insects. However, little is known about how this cooperative control is achieved during larval stages. Here, we induced silkworm superlarvae by applying the JH analogue (JHA) methoprene and used a microarray approach to survey the mRNA expression changes in response to JHA in the silkworm integument. We found that JHA application significantly increased the expression levels of most genes involved in basic metabolic processes and protein processing and decreased the expression of genes associated with oxidative phosphorylation in the integument. Several key genes involved in the pathways of insulin/insulin-like growth factor signaling (IIS) and 20E signaling were also upregulated after JHA application. Taken together, we suggest that JH may mediate the nutrient-dependent IIS pathway by regulating various metabolic pathways and further modulate 20E signaling.
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影响因子：
4.6
作者：
Kayukawa, Takumi;Tateishi, Ken;Shinoda, Tetsuro
通讯作者：
Shinoda, Tetsuro
影响因子：
11.8
作者：
Layalle, Sophie;Arquier, Nathalie;Leopold, Pierre
通讯作者：
Leopold, Pierre
DOI：
10.1016/0006-291x(78)91028-8
发表时间：
1978-01-01
影响因子：
3.1
作者：
CHEFURKA, W
通讯作者：
CHEFURKA, W
影响因子：
2.7
作者：
Koyama, Takashi;Syropyatova, Maria O.;Riddiford, Lynn M.
通讯作者：
Riddiford, Lynn M.
影响因子：
4.6
作者：
Lozano, Jesus;Belles, Xavier
通讯作者：
Belles, Xavier