Effects of Bisphosphonate Administration on Cleft Bone Graft in a Rat Model.
Effects of Bisphosphonate Administration on Cleft Bone Graft in a Rat Model.
复制标题
DOI：
10.1597/15-356
复制
发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Hong C
中科院分区：
文献类型：
--
作者：
Cheng N;Park J;Olson J;Kwon T;Lee D;Lim R;Ha S;Kim R;Zhang X;Ting K;Tetradis S;Hong C
唇腭裂患者的骨移植物会发生大量的吸收。本研究的目的是探讨双膦酸盐（BPs）对大鼠骨移植成功的影响。将35只雌性15周龄Fischer F344近交系大鼠分成以下实验组，每组接受骨移植物以修复口内CSD：（1）移植物/盐水：全身施用生理盐水和（2）手术后立即全身施用唑来膦酸（移植物/BP/T0）、（3）术后1周（移植物/BP/T1）和（4）术后3周（移植物/ BP/T2）。作为额外的对照，将缺损留空，不进行植骨。除了通过抗酒石酸酸性磷酸酶染色评价破骨细胞外，还进行了显微计算机断层扫描和组织学分析。术后6周，延迟BP治疗组的骨体积分数（骨体积/组织体积）（移植物/BP/T1 = 45.4% ± 8.8%;移植物/BP/T2 = 46.1% ± 12.4%）显著大于移植物/生理盐水组（31.0% ± 7.9%）和移植物/BP/T0（27.6% ± 5.9%）（P<0.05）。苏木素和伊红染色证实，在移植物/BP/T1和移植物/BP/T2组中，骨体积明显增加，缺损边缘与现有腭骨融合。移植物/BP/T0组显示出最低的骨体积，伴有急性炎症体征。与生理盐水对照组相比，裂骨移植手术后延迟BP给药导致骨体积和整合显著增加。然而，手术后立即注射BP并没有增加骨量，相反，可能会对植骨结合产生负面影响。
Bone grafts in patients with cleft lip and palate can undergo a significant amount of resorption. The aim of this study was to investigate the effects of bisphosphonates (BPs) on the success of bone grafts in rats. Thirty-five female 15-week-old Fischer F344 Inbred rats were divided into the following experimental groups, each receiving bone grafts to repair an intraoral CSD: (1) Graft/saline: systemic administration of saline and (2) systemic administration of zoledronic acid immediately following surgery (graft/BP/T0), (3) 1 week postoperatively (graft/BP/T1), and (4) 3 weeks postoperatively (graft/ BP/T2). As an additional control, the defect was left empty without bone graft. Microcomputed tomography and histologic analyses were performed in addition to evaluation of osteoclasts through tartrate-resistant acid phosphatase staining. Bone volume fraction (bone volume/tissue volume) for the delayed BP treatment groups (graft/BP/T1 = 45.4% ± 8.8%; graft/BP/T2 = 46.1% ± 12.4%) were significantly greater than that for the graft/saline group (31.0% ± 7.9%) and the graft/BP/T0 (27.6% ± 5.9%) 6 weeks postoperatively (P< .05). Hematoxylin and eosin staining confirmed an evident increase in bone volume and fusion of defect margins with existing palatal bone in the graft/BP/T1 and graft/BP/T2 groups. The graft/BP/T0 group showed the lowest bone volume with signs of acute inflammation. Delayed BP administration following cleft bone graft surgery led to significant increase in bone volume and integration compared with saline controls. However, BP injection immediately after the surgery did not enhance bone volume, and rather, may negatively affect bone graft incorporation.
登录
查看更多内容
DOI：
10.1002/ar.20619
发表时间：
2008-01-01
影响因子：
2
作者：
Huja, Sarandeep S.;Beck, Frank M.
通讯作者：
Beck, Frank M.
DOI：
10.1080/000164702317281350
发表时间：
2002-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
Aspenberg, P;Åstrand, J
通讯作者：
Åstrand, J
影响因子：
7.6
作者：
Grier, RL;Wise, GE
通讯作者：
Wise, GE
DOI：
10.1016/j.bjps.2015.09.004
发表时间：
2016-01-01
影响因子：
2.7
作者：
Ayoub, Ashraf;Roshan, Cherian P.;Ray, Arup
通讯作者：
Ray, Arup
DOI：
10.1016/j.coms.2015.06.001
发表时间：
2015-11
影响因子：
1.5
作者：
Aghaloo T;Hazboun R;Tetradis S
通讯作者：
Tetradis S