Autonomy of ascidian fork head/HNF-3 gene expression

Autonomy of ascidian fork head/HNF-3 gene expression
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海鞘叉头/HNF-3基因表达的自主性

DOI:
10.1016/s0925-4773(97)00162-7
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发表时间:
1997
影响因子:
2.6
通讯作者:
N. Satoh
N. Satoh
中科院分区:
生物学4区
文献类型:
--
作者:
Y. Shimauchi;H. Yasuo;N. Satoh

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我们对海鞘类 Halocynthia roretzi 的 I 类叉头/HNF-3 基因 (HrHNF3-1) 的表达模式进行了表征。合子 HrHNF3-1 表达早在 16 细胞阶段就可检测到,并且转录本在内胚层、脊索和早期胚胎的间充质谱系的卵裂球中很明显。原肠胚晚期后,HrHNF3-1也在假定的脊髓细胞和一些脑细胞中表达。海鞘蝌蚪的脊髓由四层细胞组成;背层、两个侧层和腹层,后者简单地位于脊索上。原位杂交样本的横截面显示,HrHNF3-1 在腹侧层细胞中表达,让人想起脊椎动物胚胎的底板。此外,我们发现早期 HrHNF3-1 表达的启动具有自主性,因为该基因在卵裂球中表达,从第一次卵裂一直到 16 细胞阶段持续解离。
We have characterized the expression pattern of a class I fork head/HNF-3 gene (HrHNF3-1) of the ascidian Halocynthia roretzi. Zygotic HrHNF3-1 expression was detectable as early as the 16-cell stage, and the transcript was evident in blastomeres of the endoderm, notochord and mesenchyme lineages of the early embryos. After the late gastrula stage, HrHNF3-1 was also expressed in the presumptive spinal cord cells and some brain cells. The spinal cord of the ascidian tadpole consists of four layers of cells; the dorsal layer, two lateral layers and the ventral layer, the latter of which simply lies on the notochord. Cross-sections of in situ hybridized specimens showed that HrHNF3-1 was expressed in cells of the ventral layer, reminiscent of the floor plate of vertebrate embryos. In addition, we found autonomy in the initiation of early HrHNF3-1 expression, because the gene was expressed in blastomeres continuously dissociated from the first cleavage until the 16-cell stage.
DOI: 10.5167/uzh-238
发表时间: 1996-12
期刊: Development
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DOI: 10.1006/dbio.1995.1216
发表时间: 1995
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DOI: 10.1016/s0168-9525(00)89106-4
发表时间: 1995
期刊: Trends in genetics : TIG
影响因子: --
作者:
Satoh,N;Jeffery,WR
通讯作者: Jeffery,WR
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发表时间: 1991-03-01
影响因子: 10.5
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通讯作者: DARNELL, JE
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发表时间: 1990-08-01
影响因子: 10.5
作者:
LAI, E;PREZIOSO, VR;DARNELL, JE
通讯作者: DARNELL, JE