The frequency and duration of Salmonella-macrophage adhesion events determines infection efficiency.
The frequency and duration of Salmonella-macrophage adhesion events determines infection efficiency.
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DOI：
10.1098/rstb.2014.0033
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发表时间：
2015-02-05
期刊：
影响因子：
--
通讯作者：
Bryant CE
中科院分区：
文献类型：
--
作者：
Achouri S;Wright JA;Evans L;Macleod C;Fraser G;Cicuta P;Bryant CE
肠道沙门氏菌在人类和多种动物物种中引起一系列重要疾病。细菌粘附、侵入宿主细胞并在宿主细胞内存活的能力在沙门氏菌感染的发病机制中起着重要作用。在系统性沙门氏菌病中，巨噬细胞构成宿主生物体内细菌增殖的生态位。鼠伤寒沙门氏菌是有鞭毛的，这种细菌与细胞碰撞的频率对感染效率很重要。我们研究了细菌运动性如何影响感染效率，使用群体水平的巨噬细胞感染实验和单细胞感染事件的直接成像相结合，比较野生型和运动突变体。与野生型细菌相反，非运动和无鞭毛细菌菌株与巨噬细胞碰撞的频率较低，与细胞接触的时间较短，感染的频率较低。运行偏向沙门氏菌与巨噬细胞的碰撞频率也较低，但与野生型菌株相比，与巨噬细胞保持接触的时间更长，更容易感染细胞。我们的研究结果表明，S。除了细菌与细胞碰撞的频率之外，巨噬细胞对鼠伤寒沙门氏菌的清除还取决于细菌与细胞接触的持续时间。
Salmonella enterica causes a range of important diseases in humans and a in a variety of animal species. The ability of bacteria to adhere to, invade and survive within host cells plays an important role in the pathogenesis of Salmonella infections. In systemic salmonellosis, macrophages constitute a niche for the proliferation of bacteria within the host organism. Salmonella enterica serovar Typhimurium is flagellated and the frequency with which this bacterium collides with a cell is important for infection efficiency. We investigated how bacterial motility affects infection efficiency, using a combination of population-level macrophage infection experiments and direct imaging of single-cell infection events, comparing wild-type and motility mutants. Non-motile and aflagellate bacterial strains, in contrast to wild-type bacteria, collide less frequently with macrophages, are in contact with the cell for less time and infect less frequently. Run-biased Salmonella also collide less frequently with macrophages but maintain contact with macrophages for a longer period of time than wild-type strains and infect the cells more readily. Our results suggest that uptake of S. Typhimurium by macrophages is dependent upon the duration of contact time of the bacterium with the cell, in addition to the frequency with which the bacteria collide with the cell.
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鼠伤寒沙门氏菌通过穿透和破坏佩耶斑块的专门上皮细胞来引起鼠的感染。
影响因子：
15.3
作者：
Jones, Bradley D.;Ghori, Nafisa;Falkow, Stanley
通讯作者：
Falkow, Stanley
影响因子：
3.1
作者：
Raffatellu, M;Wilson, RP;Bäumler, AJ
通讯作者：
Bäumler, AJ
影响因子：
3.2
作者：
Ohnishi, K;Fan, F;Macnab, RM
通讯作者：
Macnab, RM
影响因子：
3.4
作者：
Paul, Debjani;Achouri, Sarra;Cicuta, Pietro
通讯作者：
Cicuta, Pietro
影响因子：
3.4
作者：
Ibarra JA;Steele-Mortimer O
通讯作者：
Steele-Mortimer O