Four different methods comparison for extraction of astaxanthin from green alga Haematococcus pluvialis.
Four different methods comparison for extraction of astaxanthin from green alga Haematococcus pluvialis.
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DOI：
10.1155/2014/694305
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发表时间：
2014
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Dong S;Huang Y;Zhang R;Wang S;Liu Y
雨生红球藻是一种高效的产虾青素微生物。由于虾青素细胞壁较厚，阻碍了其溶剂提取，目前尚无有效的方法。本研究采用盐酸预处理-丙酮提取法（HCl-ACE）、正己烷/异丙醇（6：4，v/v）混合溶剂提取法（HEX-IPA）、甲醇提取-丙酮提取法（MET-ACE，两步提取法）和大豆油提取法对红球藻中虾青素的提取进行了研究。雨生植物结果表明，HCl-ACE法的出油率和虾青素含量最高，分别为33.3 ± 1.1%和19.8 ± 1.1%。定量NMR分析提供了总脂质提取物的脂肪酸链谱。在所有情况下，油烯基链占主导地位，观察到大量的多不饱和脂肪酸链，主要脂肪酸组分为油酸（13-35%）、亚油酸（37-43%）、亚麻酸（20-31%）和总饱和酸（17-28%）。HCl-ACE法提取物清除DPPH自由基的活性最高，为73.2 ± 1.0%。同时考察了四种提取方法所得提取物的还原能力。可以得出结论，在这项工作中提出的提取方法的盐酸ACE允许有效的虾青素提取具有高的抗氧化性能。
Haematococcus pluvialis is one of the potent organisms for production of astaxanthin. Up to now, no efficient method has been achieved due to its thick cell wall hindering solvent extraction of astaxanthin. In this study, four different methods, hydrochloric acid pretreatment followed by acetone extraction (HCl-ACE), hexane/isopropanol (6 : 4, v/v) mixture solvents extraction (HEX-IPA), methanol extraction followed by acetone extraction (MET-ACE, 2-step extraction), and soy-oil extraction, were intensively evaluated for extraction of astaxanthin from H. pluvialis. Results showed that HCl-ACE method could obtain the highest oil yield (33.3 ± 1.1%) and astaxanthin content (19.8 ± 1.1%). Quantitative NMR analysis provided the fatty acid chain profiles of total lipid extracts. In all cases, oleyl chains were predominant, and high amounts of polyunsaturated fatty acid chains were observed and the major fatty acid components were oleic acid (13–35%), linoleic acid (37–43%), linolenic acid (20–31%), and total saturated acid (17–28%). DPPH radical scavenging activity of extract obtained by HCl-ACE was 73.2 ± 1.0%, which is the highest amongst the four methods. The reducing power of extract obtained by four extraction methods was also examined. It was concluded that the proposed extraction method of HCl-ACE in this work allowed efficient astaxanthin extractability with high antioxidant properties.
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