Matrine induces cell cycle arrest and apoptosis with recovery of the expression of miR-126 in the A549 non-small cell lung cancer cell line.
Matrine induces cell cycle arrest and apoptosis with recovery of the expression of miR-126 in the A549 non-small cell lung cancer cell line.
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DOI：
10.3892/mmr.2016.5753
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发表时间：
2016-11
影响因子：
3.4
通讯作者：
Kan Q
中科院分区：
文献类型：
--
作者：
An Q;Han C;Zhou Y;Li F;Li D;Zhang X;Yu Z;Duan Z;Kan Q
非小细胞肺癌（NSCLC）是美国癌症相关死亡的主要原因。化疗可以延长晚期疾病患者的生存率，然而，这是以临床上显着的不良反应为代价的。苦参碱是中药的活性成分，是治疗NSCLC的有前途的替代药物。在本研究中，研究了苦参碱对 A549 NSCLC 细胞系的治疗作用及其潜在分子机制。高浓度苦参碱（1.0 mg/ml）显着（P<0.05）抑制细胞增殖，抑制率达52.68±3.32%，并观察到细胞皱缩和破裂。流式细胞分析显示，苦参碱处理48 h后，G1/G0细胞比例显着增加，而S和G2/M细胞比例显着降低（P<0.05）。这些结果表明细胞停滞是由苦参碱诱导的。使用逆转录定量聚合酶链反应分析、免疫组织化学和蛋白质印迹分析，检测到低浓度苦参碱（0.2 mg/ml）处理后，microRNA (miR)-126 表达上调，随后其靶基因血管内皮生长因子表达下调。总之，苦参碱诱导细胞周期停滞和细胞凋亡，并恢复 A549 NSCLC 细胞系中 miR-126 的表达。
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-associated mortality in the United States. Chemotherapy prolongs survival rates among patients with advanced disease, however, this is at the cost of clinically significant adverse effects. Matrine is an active component of traditional Chinese medicine and is a promising alternative drug for the treatment of NSCLC. In the present study, the therapeutic effects and the underlying molecular mechanisms of matrine on the A549 NSCLC cell line were investigated. A high concentration of matrine (1.0 mg/ml) significantly (P<0.05) inhibited cell proliferation, by 52.68±3.32%, under which cell shrinkage and disruption were observed. Flow cytometric analysis showed that the proportion of G1/G0 cells was significantly increased, whereas the proportions of S and G2/M cells were significantly decreased (P<0.05) following treatment with matrine for 48 h. These results indicated that cell arrest was induced by matrine. Upregulation of the expression of microRNA (miR)-126, followed by downregulation of the expression of its target gene, vascular endothelial growth factor, were detected following treatment with a low concentration of matrine (0.2 mg/ml) using reverse transcription-quantitative polymerase chain reaction analysis, immunohistochemistry and western blot analysis. In conclusion, matrine induced cell cycle arrest and apoptosis, and recovered the expression of miR-126 in the A549 NSCLC cell line.
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影响因子：
4.5
作者：
Luo, Cong;Zhu, Yongliang;Huang, Jian
通讯作者：
Huang, Jian
影响因子：
3.7
作者：
Martínez-Sánchez JM;Fernández E;Fu M;Gallus S;Martínez C;Sureda X;La Vecchia C;Clancy L
通讯作者：
Clancy L
影响因子：
5.4
作者：
Ooi, LSM;Wang, H;Ooi, VEC
通讯作者：
Ooi, VEC
DOI：
10.1186/1756-9966-28-112
发表时间：
2009-08-12
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Wu P;Dugoua JJ;Eyawo O;Mills EJ
通讯作者：
Mills EJ
影响因子：
5
作者：
Zhang, Lijun;Wang, Tingting;Wei, Lei
通讯作者：
Wei, Lei