cDNA-AFLP analysis of differentially expressed genes during microspore embryogenesis in non-heading Chinese cabbage
cDNA-AFLP analysis of differentially expressed genes during microspore embryogenesis in non-heading Chinese cabbage
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不结球白菜小孢子胚胎发生过程中差异表达基因的cDNA-AFLP分析
DOI：
10.1007/s11627-019-10036-0
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发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Li Ying
中科院分区：
文献类型：
--
作者：
Zhang Ya;Gao Suyan;Liu Huanhuan;Zhang Xilin;Zeng Aisong;Wang Jianjun;Hou Xilin;Li Ying
逆境可诱导小孢子由配子体发育途径转变为胚性发育途径。为探讨小孢子胚胎发生的分子机制，利用互补DNA扩增片段长度多态性技术分离了不结球白菜小孢子胚胎发生过程中的转录片段。SSP.五月曼通过对256对引物组合的筛选，共鉴定出94个转录产物片段，其中15个片段成功测序。基于BLAST检索，15个测序的转录衍生片段中有12个与注释基因同源;其余3个转录衍生片段与任何序列都不匹配。带有注释的转录衍生片段参与细胞壁形成、激素和抗性。对12个转录产物片段的启动子序列进行顺式元件分析，结果表明，在转录产物片段的启动子序列中存在热激相关和胁迫相关的顺式元件，并选择TDF 1（Bra 040720）、TDF 6（Bra 013664）和TDF 15（Bra 022587）进行实时定量PCR分析。结果证实了三个基因的互补DNA扩增片段长度多态性所揭示的改变的表达模式。本研究为不结球白菜小孢子胚胎发生的分子机制提供了新的信息。
Stress can induce microspores to change their developmental pathway from the gametophytic to the embryogenic pathway. To explore the molecular mechanisms of microspore embryogenesis, complement DNA-amplified fragment length polymorphism was used to isolate the transcript-derived fragments during microspore embryogenesis of the non-headingBrassica campestrisL. ssp. chinensis‘Wuyueman’. With 256 primer combinations screened, a total of 94 transcript-derived fragments were identified, and 15 were successfully sequenced. Based on a BLAST search in theBrassicadatabase, 12 of the 15 sequenced transcript-derived fragments were homologous to genes with annotations; the remaining three transcript-derived fragments did not match any sequences. Transcript-derived fragments with annotations were involved in cell wall formation, hormones, and resistance. Analysis ofcis-elements indicated that there were heat shock-related and stress-relatedcis-elements in the promoter sequences of 12 transcript-derived fragments.TDF1(Bra040720),TDF6(Bra013664), andTDF15(Bra022587) were selected for validation of complement DNA-amplified fragment length polymorphism expression patterns by real-time quantitative PCR. Results confirmed the altered expression patterns of three genes revealed by the complement DNA-amplified fragment length polymorphism. This study provides novel information on the molecular mechanism of microspore embryogenesis in non-heading Chinese cabbage.
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DOI：
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发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.9
作者：
Mulako I;Farrant JM;Collett H;Illing N
通讯作者：
Illing N
影响因子：
4.6
作者：
Li J;Huang Q;Sun M;Zhang T;Li H;Chen B;Xu K;Gao G;Li F;Yan G;Qiao J;Cai Y;Wu X
通讯作者：
Wu X
影响因子：
5.6
作者：
Liu X;Han F;Kong C;Fang Z;Yang L;Zhang Y;Zhuang M;Liu Y;Li Z;Lv H
通讯作者：
Lv H
影响因子：
2.4
作者：
M. Melloul;D. Iraqi;MyAbdelaziz El Alaoui;G. Erba;S. Alaoui;M. Ibriz;E. Elfahime
通讯作者：
M. Melloul;D. Iraqi;MyAbdelaziz El Alaoui;G. Erba;S. Alaoui;M. Ibriz;E. Elfahime