Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.
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DOI：
10.1186/1746-6148-7-42
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发表时间：
2011-08-02
影响因子：
2.6
通讯作者：
Staszyk C
中科院分区：
文献类型：
--
作者：
Mensing N;Gasse H;Hambruch N;Haeger JD;Pfarrer C;Staszyk C
马的牙周组织提供牙齿支持和终身的牙齿萌出显着规模。这些功能需要持续的组织重塑。据推测，多能间充质基质细胞（MSC）驻留在牙周膜（PDL），并在调节生理性牙周组织再生中起着至关重要的作用。本研究的目的是分离和表征马牙周MSC。组织样品从四匹健康的马中获得。从牙龈、PDL的三个水平水平（根尖、中牙和龈下）和皮下组织（咬肌区域）收获并培养原代细胞群。集落形成细胞在未涂覆的培养皿上生长，并分析非人MSC的典型体外特征，即自我更新能力、群体倍增时间、干细胞标记物的表达和三系分化。来自所有位置的集落形成细胞群体显示干细胞标记物CD 90和CD 105的表达。通过集落形成单位成纤维细胞（CFU-F）测定证明了体外自我更新能力。CFU效率是最高的细胞群从顶端和中间的牙齿PDL。群体倍增时间在皮下细胞中最高。所有研究的细胞群具有成骨、成脂和成软骨的三系分化潜能。由于所证明的体外特征，细胞被称为马皮下MSC（eSc-MSC）、马牙龈MSC（eG-MSC）和马牙周MSC（eP-MSC）。根据PDL水平的不同，eP-MSC进一步被指定为来自顶端PDL的eP-MSC（eP-MScap）、来自牙中部PDL的eP-MSC（eP-MSCm）和来自牙龈下PDL的eP-MSC（eP-MSCsg）。考虑到目前的概念，基于细胞的再生疗法在马，eP-MSC可能是有前途的候选人，为未来的临床应用在马骨科和牙周疾病。
The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the periodontal ligament (PDL) and play a crucial role in regulating physiological periodontal tissue regeneration. The aim of this study was to isolate and characterize equine periodontal MSC. Tissue samples were obtained from four healthy horses. Primary cell populations were har-vested and cultured from the gingiva, from three horizontal levels of the PDL (apical, midtooth and subgingival) and for comparison purposes from the subcutis (masseteric region). Colony-forming cells were grown on uncoated culture dishes and typical in vitro characteristics of non-human MSC, i.e. self-renewal capacity, population doubling time, expression of stemness markers and trilineage differentiation were analyzed. Colony-forming cell populations from all locations showed expression of the stemness markers CD90 and CD105. In vitro self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFU-F) assays. CFU-efficiency was highest in cell populations from the apical and from the mid-tooth PDL. Population doubling time was highest in subcutaneous cells. All investigated cell populations possessed trilineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages. Due to the demonstrated in vitro characteristics cells were referred to as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC) and equine periodontal MSC (eP-MSC). According to different PDL levels, eP-MSC were further specified as eP-MSC from the apical PDL (eP-MSCap), eP-MSC from the mid-tooth PDL (eP-MSCm) and eP-MSC from the subgingival PDL (eP-MSCsg). Considering current concepts of cell-based regenerative therapies in horses, eP-MSC might be promising candidates for future clinical applications in equine orthopedic and periodontal diseases.
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影响因子：
2.6
作者：
Basdra, EK;Komposch, G
通讯作者：
Komposch, G
影响因子：
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作者：
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Mcllwraith, C. Wayne
影响因子：
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作者：
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通讯作者：
Young, Marian F.
影响因子：
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作者：
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通讯作者：
Deffune, Elenice
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作者：
Braun, Julian;Hack, Anita;Aicher, Wilhelm K.
通讯作者：
Aicher, Wilhelm K.