Full genetic characterization and epidemiology of a novel amdoparvovirus in striped skunk (Mephitis mephitis).
Full genetic characterization and epidemiology of a novel amdoparvovirus in striped skunk (Mephitis mephitis).
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DOI：
10.1038/emi.2017.13
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发表时间：
2017-05-10
影响因子：
13.2
通讯作者：
Lang AS
中科院分区：
文献类型：
--
作者：
Canuti M;Doyle HE;P Britton A;Lang AS
Amdoparvovirus是一种新定义的细小病毒属，包含4个种（食肉动物Amdoparvovirus 1-4），包括著名的阿留申貂病病毒（AMDV）。Amdoparvoviruses在鼬科和犬科宿主中引起免疫相关的且通常致命的消耗综合征。在这项研究中，我们的分子研究发现，44条纹臭鼬（Mephitis mephitis）死亡的温哥华，不列颠哥伦比亚省，加拿大。一些动物表现出与细小病毒相关疾病相符的病理变化。获得了七种不同毒株的几乎完整的基因组序列，我们的分析显示了我们命名为臭鼬Amdoparvovirus（SKAV）的这种病毒应如何被归类为属内的一个单独物种（提出的食肉动物Amdoparvovirus 5）。我们检测到合并感染，重组基因组，至少三个独立的病毒谱系，和谱系的地理隔离的初步证据。此外，我们证明了类似的病毒，只有部分特征在以前的研究和标记为AMDV，在臭鼬从北美其他遥远的地区（安大略和加州），并发现在水貂（Neovison vison）的溢出事件的证据。虽然SKAV能够在受感染的动物中引起疾病，但已观察到高比例的亚临床感染，这表明这些动物可能作为无症状的携带者，并对野生和圈养的食肉动物构成威胁。最后，我们强调需要进行更具体的诊断测试和进一步的分子研究，以阐明氨多细小病毒在陆地食肉动物中的流行病学以及宿主和地理分布，特别是因为该群体中的整个病毒多样性可能仍然未知。
Amdoparvovirus is a newly defined parvoviral genus that contains four species (Carnivore amdoparvovirus 1–4), including the well-known Aleutian mink disease virus (AMDV). Amdoparvoviruses cause an immune-associated and often lethal wasting syndrome in Mustelidae and Caninae hosts. In this study, we molecularly investigated amdoparvoviruses detected in 44 striped skunks (Mephitis mephitis) found dead in and around Vancouver, British Columbia, Canada. Some of the animals exhibited pathological changes compatible with amdoparvovirus-associated disease. The nearly complete genomic sequence was obtained for seven different strains and our analyses show how this virus, which we named skunk amdoparvovirus (SKAV), should be classified as a separate species within the genus (proposed Carnivore amdoparvovirus 5). We detected co-infections, recombinant genomes, at least three separate viral lineages, and preliminary evidence for geographic segregation of lineages. Furthermore, we proved that similar viruses, only partially characterized in previous studies and labeled as AMDV, circulate in skunks from other distant areas of North America (Ontario and California) and found evidence for spillover events in mink (Neovison vison). Although SKAVs are capable of causing disease in infected animals, a high proportion of sub-clinical infections has been observed, suggesting these animals might act as asymptomatic carriers and pose a threat to wild and captive carnivores. Finally, we highlight the need for more specific diagnostic tests and further molecular investigations to clarify the epidemiology and host- and geographical distributions of amdoparvoviruses in terrestrial carnivores, especially because the whole spectrum of viral diversity in this group is likely still unknown.
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影响因子：
4.8
作者：
Bodewes R;Ruiz-Gonzalez A;Schapendonk CM;van den Brand JM;Osterhaus AD;Smits SL
通讯作者：
Smits SL
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
2.1
作者：
Farid AH
通讯作者：
Farid AH
影响因子：
4.3
作者：
Britton, A. P.;Redford, T.;Joseph, T.
通讯作者：
Joseph, T.
影响因子：
1.3
作者：
Nituch, Larissa A.;Bowman, Jeff;Schulte-Hostedde, Albrecht I.
通讯作者：
Schulte-Hostedde, Albrecht I.