The lower airways microbiome and antimicrobial peptides in idiopathic pulmonary fibrosis differ from chronic obstructive pulmonary disease.
The lower airways microbiome and antimicrobial peptides in idiopathic pulmonary fibrosis differ from chronic obstructive pulmonary disease.
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特发性肺纤维化中的下呼吸道微生物组和抗菌肽与慢性阻塞性肺疾病不同。
DOI：
10.1371/journal.pone.0262082
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Eagan TM
中科院分区：
文献类型：
--
作者：
Knudsen KS;Lehmann S;Nielsen R;Tangedal S;Haaland I;Hiemstra PS;Eagan TM
慢性肺部疾病中的下呼吸道微生物组和宿主免疫反应尚不完全清楚。我们的目的是研究特发性肺纤维化（IPF）和慢性阻塞性肺疾病（COPD）中可能的微生物组特征以及关键抗菌肽和蛋白质。通过口腔冲洗 (OW)、受保护的支气管肺泡灌洗 (PBAL) 和右肺受保护的无菌刷洗 (rPSB) 对 12 名 IPF 患者、12 名 COPD 患者和 12 名健康对照进行取样。通过酶联免疫吸附测定 (ELISA) 测量 PBAL 中的抗菌肽和蛋白质 (AMP)、分泌性白细胞蛋白酶抑制剂 (SLPI) 以及人 β 防御素 1 和 2 (hBD-1 和 hBD-2)。对细菌 16S rDNA 基因的 V3V4 区域进行了测序。使用 QIIME 2 进行生物信息分析。与 COPD 相比，IPF PBAL 中的 hBD-1 水平较低。 IPF的主要门是厚壁菌门、拟杆菌门和放线菌门；变形菌是慢性阻塞性肺病的前三名。属水平的差异丰度分析显示，研究组之间丰度较低的微生物（主要是口咽微生物）存在显着差异。与 COPD (p = 0.03) 和对照 (p = 0.01) 相比，PBAL 中 IPF 的 Alpha 多样性较低，与 COPD (p = 0.02) 和对照 (p = 0.04) 相比，rPSB 中的 Alpha 多样性较低。与 COPD 和对照相比，IPF 的系统发育 beta 多样性显示出显着更高的相似性。 α 多样性和 AMP 之间没有显着相关性。 IPF 的微生物多样性与 COPD 和对照不同，同时抗菌肽也存在差异。 IPF 中 OW 和 PBAL 之间的 Beta 多样性相似性可能表明微抽吸有助于其微生物组的变化。
The lower airways microbiome and host immune response in chronic pulmonary diseases are incompletely understood. We aimed to investigate possible microbiome characteristics and key antimicrobial peptides and proteins in idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). 12 IPF patients, 12 COPD patients and 12 healthy controls were sampled with oral wash (OW), protected bronchoalveolar lavage (PBAL) and right lung protected sterile brushings (rPSB). The antimicrobial peptides and proteins (AMPs), secretory leucocyte protease inhibitor (SLPI) and human beta defensins 1 and 2 (hBD-1 & hBD-2), were measured in PBAL by enzyme linked immunosorbent assay (ELISA). The V3V4 region of the bacterial 16S rDNA gene was sequenced. Bioinformatic analyses were performed with QIIME 2. hBD-1 levels in PBAL for IPF were lower compared with COPD. The predominant phyla in IPF were Firmicutes, Bacteroides and Actinobacteria; Proteobacteria were among top three in COPD. Differential abundance analysis at genus level showed significant differences between study groups for less abundant, mostly oropharyngeal, microbes. Alpha diversity was lower in IPF in PBAL compared to COPD (p = 0.03) and controls (p = 0.01), as well as in rPSB compared to COPD (p = 0.02) and controls (p = 0.04). Phylogenetic beta diversity showed significantly more similarity for IPF compared with COPD and controls. There were no significant correlations between alpha diversity and AMPs. IPF differed in microbial diversity from COPD and controls, accompanied by differences in antimicrobial peptides. Beta diversity similarity between OW and PBAL in IPF may indicate that microaspiration contributes to changes in its microbiome.
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影响因子：
8.3
作者：
Dickson, Robert P.;Erb-Downward, John R.;Curtis, Jeffrey L.
通讯作者：
Curtis, Jeffrey L.
DOI：
10.1164/rccm.202002-0460oc
发表时间：
2021-02-01
影响因子：
24.7
作者：
Invernizzi R;Wu BG;Barnett J;Ghai P;Kingston S;Hewitt RJ;Feary J;Li Y;Chua F;Wu Z;Wells AU;George PM;Renzoni EA;Nicholson AG;Rice A;Devaraj A;Segal LN;Byrne AJ;Maher TM;Lloyd CM;Molyneaux PL
通讯作者：
Molyneaux PL
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
18.2
作者：
Dickson RP;Erb-Downward JR;Martinez FJ;Huffnagle GB
通讯作者：
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