Wharton's Jelly Transplantation Improves Neurologic Function in a Rat Model of Traumatic Brain Injury.
Wharton's Jelly Transplantation Improves Neurologic Function in a Rat Model of Traumatic Brain Injury.
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沃顿商学院的果冻移植改善了创伤性脑损伤大鼠模型的神经功能
DOI：
10.1007/s10571-015-0159-9
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发表时间：
2015-07
影响因子：
4
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Cheng, Tian;Yang, Bo;Li, Dongpeng;Ma, Shanshan;Tian, Yi;Qu, Ruina;Zhang, Wenjin;Zhang, Yanting;Hu, Kai;Guan, Fangxia;Wang, Jian
创伤性脑损伤（TBI）可导致残疾、功能障碍甚至死亡，是世界范围内一个突出的健康问题。这种严重且令人衰弱的疾病需要有效的治疗。人类脐带基质，称为沃顿胶 (WJ)，提供了富含间充质干细胞的天然界面支架。在这项研究中，我们测试了 WJ 组织移植在大鼠 TBI 体重下降模型中的功效。 TBI后24小时培养WJ组织并移植到损伤部位。在 TBI 后的不同时间点评估改良的神经系统严重程度评分、体重、脑水肿和病变体积。通过新物体识别测试和莫里斯水迷宫测试评估认知行为。 TBI 后第 14 天测量病灶周围脑区脑源性神经营养因子 (BDNF) 的表达。我们发现 WJ 组织移植减轻了 TBI 引起的脑水肿（第 3 天），缩小了病变体积（第 28 天），改善了神经功能（第 21 至 28 天），并促进了记忆和认知恢复。此外，WJ 组织治疗的大鼠中 BDNF mRNA 和蛋白的表达高于假手术或载体治疗的大鼠。这些数据表明WJ组织移植可以减少TBI引起的脑损伤，并且可能具有治疗TBI的治疗潜力。
Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton's jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21 to 28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI.
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影响因子：
6.1
作者：
Wu, He;Wu, Tao;Li, Mingchang;Wang, Jian
通讯作者：
Wang, Jian
侧向头部加速性脑损伤大鼠海马中S100A6的表达
影响因子：
5.6
作者：
Fang B;Liang M;Yang G;Ye Y;Xu H;He X;Huang JH
通讯作者：
Huang JH
不同强度的跑步机运动对大鼠严重受控皮质冲击后认知功能缺陷的影响
影响因子：
5.6
作者：
Shen X;Li A;Zhang Y;Dong X;Shan T;Wu Y;Jia J;Hu Y
通讯作者：
Hu Y
影响因子：
4.3
作者：
Zhou, Changhui;Yang, Bo;Tian, Yi;Jiao, Hongliang;Zheng, Wendi;Wang, Jian;Guan, Fangxia
通讯作者：
Guan, Fangxia
影响因子：
1.7
作者：
Malkowski, Andrzej;Sobolewski, Krzysztof;Bankowski, Edward
通讯作者：
Bankowski, Edward