Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo.
Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo.
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DOI：
10.1242/dev.016329
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发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
Coonrod SA
中科院分区：
文献类型：
--
作者：
Yurttas P;Vitale AM;Fitzhenry RJ;Cohen-Gould L;Wu W;Gossen JA;Coonrod SA
哺乳动物卵细胞向胚胎过渡过程中全能性建立的机制尚不清楚。细胞质晶格（cpl）是哺乳动物卵母细胞和着床前胚胎所特有的结构，长期以来一直被预测为母体对早期胚胎的核糖体和mRNA的储存形式。在这里，我们证明了卵母细胞和胚胎限制性肽精氨酸脱亚胺酶6 （PADI6）是将核糖体成分并入卵母细胞cpl所必需的。接下来，我们发现核糖体成分的丰度和定位在PADI6-/-双细胞胚胎中受到显著影响，并且这些胚胎中的新生蛋白质合成也受到失调。最后，我们证明胚胎基因组激活（EGA）在PADI6 -/-双细胞胚胎中存在缺陷。这些结果表明，在哺乳动物中，核糖体成分储存在卵母细胞cpl中，并且是早期发育过程中蛋白质翻译所必需的。
The mechanisms mediating the establishment of totipotency during the egg-to-embryo transition in mammals remain poorly understood. The cytoplasmic lattices (CPLs), a structure unique to mammalian oocytes and preimplantation embryos, have long been predicted to function as a storage form for the maternal contribution of ribosomes and mRNA to the early embryo. Here, we demonstrate that oocyte- and embryo-restricted peptidylarginine deiminase 6 (PADI6) is required for incorporation of ribosomal components into the oocyte CPLs. Next, we show that the abundance and localization of ribosomal components is dramatically affected in PADI6-/- two-cell embryos and that de novo protein synthesis is also disregulated in these embryos. Finally, we demonstrate that embryonic genome activation (EGA) is defective in PADI6 -/- two-cell embryos. These results suggest that, in mammals, ribosomal components are stored in the oocyte CPLs and are required for protein translation during early development.
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