Full-Genome Sequencing as a Basis for Molecular Epidemiology Studies of Bluetongue Virus in India.
Full-Genome Sequencing as a Basis for Molecular Epidemiology Studies of Bluetongue Virus in India.
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DOI：
10.1371/journal.pone.0131257
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Mertens PP
中科院分区：
文献类型：
--
作者：
Maan S;Maan NS;Belaganahalli MN;Rao PP;Singh KP;Hemadri D;Putty K;Kumar A;Batra K;Krishnajyothi Y;Chandel BS;Reddy GH;Nomikou K;Reddy YN;Attoui H;Hegde NR;Mertens PP
自1998年以来，蓝舌病病毒（BTV）的全球分布发生了重大变化。在欧洲已经检测到10种以前外来的BTV血清型，在幼稚反刍动物群体中引起严重的疾病暴发。以前外来的BTV血清型也在美国、以色列、澳大利亚和印度被鉴定。BTV通过叮咬蠓（库蠓属）传播。病媒物种分布的变化、气候变化、国际旅行和贸易的增加被认为是造成这些事件的原因。自1964年印度首次报告该病以来，已在印度分离出13种BTV血清型。用于制备病毒dsRNA和cDNA合成的有效方法促进了来自该区域的BTV毒株的全基因组测序。这些研究为BTV的鉴定提供了一种新的方法，基于全基因组测序和系统发育分析，有助于BTV血清型、拓扑型和抗性株的鉴定。系统发育分析表明，印度BTV株的大多数等效基因组片段是密切相关的，聚集在一个主要的东部BTV“拓扑型”。然而，基因组片段5（Seg-5）编码NS 1，从多个1982年后的印度分离株，起源于西部BTV拓扑型。BTV-2分离株（IND 2003/01、IND 2003/02和IND 2003/03）的所有10个基因组片段与南非BTV-2疫苗株（西部拓扑型）密切相关（同一性>99%）。类似地，BTV-10分离株（IND 2003/06; IND 2005/04）与来自美国的原型BTV-10（CA-8）株在所有基因组区段中显示>99%的同一性。这些数据表明，西方BTV田间和/或疫苗株反复引入印度，可能与动物或媒介昆虫运动有关，或未经授权在该国使用“活”南非或美国BTV疫苗。作为控制策略的一部分，所提供的数据将有助于改善基于核酸的印度血清型/拓扑型诊断。
Since 1998 there have been significant changes in the global distribution of bluetongue virus (BTV). Ten previously exotic BTV serotypes have been detected in Europe, causing severe disease outbreaks in naïve ruminant populations. Previously exotic BTV serotypes were also identified in the USA, Israel, Australia and India. BTV is transmitted by biting midges (Culicoides spp.) and changes in the distribution of vector species, climate change, increased international travel and trade are thought to have contributed to these events. Thirteen BTV serotypes have been isolated in India since first reports of the disease in the country during 1964. Efficient methods for preparation of viral dsRNA and cDNA synthesis, have facilitated full-genome sequencing of BTV strains from the region. These studies introduce a new approach for BTV characterization, based on full-genome sequencing and phylogenetic analyses, facilitating the identification of BTV serotype, topotype and reassortant strains. Phylogenetic analyses show that most of the equivalent genome-segments of Indian BTV strains are closely related, clustering within a major eastern BTV ‘topotype’. However, genome-segment 5 (Seg-5) encoding NS1, from multiple post 1982 Indian isolates, originated from a western BTV topotype. All ten genome-segments of BTV-2 isolates (IND2003/01, IND2003/02 and IND2003/03) are closely related (>99% identity) to a South African BTV-2 vaccine-strain (western topotype). Similarly BTV-10 isolates (IND2003/06; IND2005/04) show >99% identity in all genome segments, to the prototype BTV-10 (CA-8) strain from the USA. These data suggest repeated introductions of western BTV field and/or vaccine-strains into India, potentially linked to animal or vector-insect movements, or unauthorised use of ‘live’ South African or American BTV-vaccines in the country. The data presented will help improve nucleic acid based diagnostics for Indian serotypes/topotypes, as part of control strategies.
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影响因子：
64.8
作者：
Grimes, JM;Burroughs, JN;Stuart, DI
通讯作者：
Stuart, DI
影响因子：
4.3
作者：
Desai, G. S.;Hosamane, M.;Mondal, B.
通讯作者：
Mondal, B.
影响因子：
3.7
作者：
Batten C;Darpel K;Henstock M;Fay P;Veronesi E;Gubbins S;Graves S;Frost L;Oura C
通讯作者：
Oura C
影响因子：
3.7
作者：
Anthony, S. J.;Darpel, K. E.;Mertens, P. P. C.
通讯作者：
Mertens, P. P. C.
DOI：
10.1016/b978-012369368-6.50007-1
发表时间：
2009-01-01
期刊：
BLUETONGUE
影响因子：
--
作者：
Attoui, Houssam;Maan, Sushila;Mertens, Peter P. C.
通讯作者：
Mertens, Peter P. C.