Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii.
Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii.
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微藻莱茵衣藻不同营养物质的生长和脂质积累
DOI：
10.1186/s13068-018-1041-z
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发表时间：
2018
影响因子：
6.3
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Yang L;Chen J;Qin S;Zeng M;Jiang Y;Hu L;Xiao P;Hao W;Hu Z;Lei A;Wang J
背景个体营养消耗被广泛用于诱导微藻中的脂质积累，这也会导致细胞生长抑制并降低总生物量。因此，在营养缺乏细胞中改善脂质积累而不损失生物量成为廉价生物燃料的一种潜在的、经济有效的治疗方法。方法在本研究中，比较了不同营养条件对莱茵衣藻生长和脂质含量的影响，并研究了不同营养条件下的代谢特征。结果我们发现，与其他微藻类似，氮或磷缺乏抑制衣藻的生长，复合营养缺乏使生物量减少高达31.7%，尽管细胞中的脂质含量（g/g干重[DW]）显着增加。添加乙酸钠可以抵消由于氮和磷缺乏而导致的生长抑制，并显着增加生物量。此外，与营养耗尽和正常培养条件相比，补充 4 g/L 乙酸钠与氮和磷缺乏的组合使总脂肪酸产量 (mg/L) 分别增加了 93.0 和 150.1%。代谢物含量受不同营养条件的影响，特别是参与脂质代谢、氨基酸代谢和外界物质代谢的代谢物。结论对这些代谢物的进一步研究可以揭示衣藻细胞生长抑制与脂肪酸积累之间的关系。
BackgroundIndividual nutrient depletion is widely used to induce lipid accumulation in microalgae, which also causes cell growth inhibition and decreases the total biomass. Thus, improving the lipid accumulation without biomass loss in the nutrient deficiency cells becomes a potential cost-effective treatment for cheaper biofuels.MethodsIn this study, the effects of different nutritional conditions on the growth and contents of lipids in Chlamydomonas reinhardtii were compared, and the metabolic profiles under different nutritional conditions were also investigated.ResultsWe showed that similar to other microalgae, nitrogen or phosphorus deficiency inhibited the growth of Chlamydomonas and combined nutrition deficiency reduced biomass by up to 31.7%, though lipid contents in cells (g/g dry weight [DW]) were significantly increased. The addition of sodium acetate countered this growth inhibition that resulted from nitrogen and phosphorus deficiency, with significantly increased biomass. Furthermore, the combination of 4 g/L sodium acetate supplementation with nitrogen and phosphorous deficiency increased total fatty acid yield (mg/L) by 93.0 and 150.1% compared to nutrient-depleted and normal culture conditions, respectively. Metabolite content was affected by the different nutritional conditions, especially metabolites that are involved in lipid metabolism, amino acid metabolism and metabolism of external substances.ConclusionFurther research into these metabolites could shed light onto the relationship between cell growth inhibition and fatty acid accumulation in Chlamydomonas.
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影响因子：
6.3
作者：
Ho SH;Nakanishi A;Ye X;Chang JS;Chen CY;Hasunuma T;Kondo A
通讯作者：
Kondo A
影响因子：
3.7
作者：
Popko J;Herrfurth C;Feussner K;Ischebeck T;Iven T;Haslam R;Hamilton M;Sayanova O;Napier J;Khozin-Goldberg I;Feussner I
通讯作者：
Feussner I
DOI：
10.1016/j.cep.2009.03.006
发表时间：
2009-06-01
影响因子：
4.3
作者：
Converti, Attilio;Casazza, Alessandro A.;Del Borghi, Marco
通讯作者：
Del Borghi, Marco
影响因子：
3
作者：
Chen, Taojing;Zhao, Quanyu;Wei, Wei
通讯作者：
Wei, Wei
影响因子：
10.4
作者：
Demirbas, Ayhan;Demirbas, M. Fatih
通讯作者：
Demirbas, M. Fatih