Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting.

Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting.
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DOI:
10.1051/parasite/2013017
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发表时间:
2013
期刊:
Parasite (Paris, France)
影响因子:
--
通讯作者:
Mohebali M
Mohebali M
中科院分区:
其他
文献类型:
--
作者:
Ali-Heydari S;Keshavarz H;Shojaee S;Mohebali M

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为进行IgG亲合力免疫印迹试验检测急性弓形虫病,从伊朗德黑兰采集了100份血清样品。弓形虫特异性IgG和IgM抗体的存在通过商业Trinity试剂盒检查。根据IgG亲合力ELISA将样品分为弓形虫感染的急性期和慢性期。进行IgG亲合力免疫印迹,分子量为22、25、28、30、32、42、44、49、55、60、66、69、88、106、130和157 kDa的抗原条带被识别为低亲合力标记物。低亲合力的最普遍抗原是p22。结论:IgG亲合力免疫印迹法可区分T细胞急性期和慢性期。弓形虫感染
To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of T. gondii infection.
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