IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans.
IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans.
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DOI:
10.3347/kjp.2012.50.2.99
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发表时间:
2012-06
期刊:
影响因子:
--
通讯作者:
Rezaeian M
中科院分区:
文献类型:
--
作者:
Rahbari AH;Keshavarz H;Shojaee S;Mohebali M;Rezaeian M
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI≤50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
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影响因子:
6.4
作者:
HEDMAN, K;LAPPALAINEN, M;MAKELA, O
通讯作者:
MAKELA, O
影响因子:
9.1
作者:
HEDMAN, K;SEPPALA, I
通讯作者:
SEPPALA, I
DOI:
10.1590/s0036-46652008000400010
发表时间:
2008-08-01
期刊:
Revista do Instituto de Medicina Tropical de São Paulo
影响因子:
--
作者:
Leite, Marcel;Siciliano, Sonia;Granato, Celso F. H.
通讯作者:
Granato, Celso F. H.
影响因子:
9.4
作者:
Montoya, JG;Huffman, HB;Remington, JS
通讯作者:
Remington, JS
DOI:
10.1128/cdli.7.4.703-705.2000
发表时间:
2000-07-01
期刊:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子:
--
作者:
Mechain, B;Garin, YJF;Derouin, F
通讯作者:
Derouin, F