IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans.

IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans.
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DOI:
10.3347/kjp.2012.50.2.99
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发表时间:
2012-06
期刊:
The Korean journal of parasitology
影响因子:
--
通讯作者:
Rezaeian M
Rezaeian M
中科院分区:
其他
文献类型:
--
作者:
Rahbari AH;Keshavarz H;Shojaee S;Mohebali M;Rezaeian M

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从伊朗德黑兰的不同实验室收集血清样本,总数为 100 份,并使用间接免疫荧光抗体测试 (IFAT) 测试抗弓形虫特异性 IgG 和 IgM 抗体。使用 IgG(慢性)和 IgM(急性)阳性样本,通过 ELISA 在两排 96 孔微量滴定板中进行 IgG 亲和力测试。一行用 6 M 尿素洗涤,另一行用 PBS (pH 7.2) 洗涤,然后计算亲合力指数 (AI)。 18份急性弓形体病血清中有16份(88.9%)呈低亲和力指数(AI≤50),82份慢性感染血清中有76份(92.7%)呈高亲和力指数(AI>60)。六种血清的人工智能水平处于临界范围。结果表明,ELISA法检测IgG亲和力可以区分人类弓形虫病的急性期和慢性期。
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI≤50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
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