In Vitro and In Vivo Antitrypanosomal Activities of Methanol Extract of Echinops kebericho Roots.
In Vitro and In Vivo Antitrypanosomal Activities of Methanol Extract of Echinops kebericho Roots.
复制标题
DOI：
10.1155/2020/8146756
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Abay SM
中科院分区：
文献类型：
--
作者：
Abdeta D;Kebede N;Giday M;Terefe G;Abay SM
微生物对几种常规抗锥虫药物的耐药性、媒介对杀虫剂的耐药性增加、缺乏有效疫苗以及现有抗锥虫药物的不良反应证明迫切需要有效、可耐受和负担得起的药物。我们评估的抗锥虫效果的水甲醇提取物的Echinops kebericho Mesfin根对锥虫刚果现场分离使用在体外和体内技术。评估了瑞士白化病小鼠的寄生虫负荷、红细胞压积（PCV）、体重和直肠温度。这一发现是被忽视的热带病药物发现研究成果的一部分。在4和2 mg/mL浓度下，提取物在40 min内阻止了锥虫的运动，而在未处理的对照中，运动持续超过160 min。与对照组相比，该提取物还显著降低寄生虫血症并防止PCV和体重的下降（p < 0.05）。植物化学分析表明，黄酮类化合物，三萜，类固醇，皂苷，糖苷，单宁和生物碱的存在。观察到该提取物具有抗寄生虫的活性。需要分离和纯化特定化合物以鉴定负责所研究药用植物的抗锥虫活性的命中化合物。
Microbial resistance to the few conventional antitrypanosomal drugs, increasing resistance of vectors to insecticides, lack of effective vaccines, and adverse effects of the existing antitrypanosomal drugs justify the urgent need for effective, tolerable, and affordable drugs. We assessed antitrypanosomal effects of the hydromethanolic extract of Echinops kebericho Mesfin roots against Trypanosoma congolense field isolate using in vitro and in vivo techniques. Parasite load, packed cell volume (PCV), body weight, and rectal temperature in Swiss albino mice were assessed. This finding is part of the outcomes of drug discovery research for neglected tropical diseases. The extract arrested the motility of trypanosomes within 40 min at 4 and 2 mg/mL concentration, whereas in the untreated control, motility continued for more than 160 min. The extract also reduced parasitemia and prevented drop in PCV and body weight significantly (p < 0.05), as compared to control. Phytochemical analysis showed the presence of flavonoids, triterpenes, steroids, saponins, glycosides, tannins, and alkaloids. It is observed that this extract has activity against the parasite. Isolation and purification of specific compounds are required to identify hit compounds responsible for the antitrypanosomal activity of the studied medicinal plant.
登录
查看更多内容
影响因子：
1.9
作者：
Yabu, Y;Yoshida, A;Ohta, N
通讯作者：
Ohta, N
影响因子：
5.4
作者：
Freiburghaus, F;Kaminsky, R;Brun, R
通讯作者：
Brun, R
影响因子：
2.1
作者：
HERBERT, WJ;LUMSDEN, WHR
通讯作者：
LUMSDEN, WHR
影响因子：
2.6
作者：
Degneh, Efrem;Ashenafi, Hagos;Terefe, Getachew
通讯作者：
Terefe, Getachew
DOI：
10.4314/ajtcam.v8i5s.23
发表时间：
2011-01-01
影响因子：
--
作者：
Nweze, N. E.;Anene, B. M.;Asuzu, I. U.
通讯作者：
Asuzu, I. U.