Expression of ethylene response factor JERF1 in rice improves tolerance to drought
Expression of ethylene response factor JERF1 in rice improves tolerance to drought
复制标题
乙烯反应因子JERF1在水稻中的表达提高了对干旱的耐受性
DOI：
10.1007/s00425-010-1208-8
复制
发表时间：
2010-06
期刊：
影响因子：
4.3
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li F;Li D;Huang R;Zhang H;Zhang Z
乙烯反应因子（ERF）蛋白调节植物的多种胁迫反应。番茄ERF蛋白JERF 1可被脱落酸（阿坝）诱导表达。过量表达JERF 1可通过与DRE/CRT和GCC-box顺式元件结合调节胁迫响应基因的表达，增强转基因烟草对高盐、渗透胁迫和低温的耐受性。在本研究中，我们进一步报道了JERF 1的过表达显著增强了转基因水稻的耐旱性。过量表达激活了胁迫响应基因的表达，并通过调节编码脯氨酸生物合成关键酶δ-吡咯啉-5-羧酸合成酶的OsP 5CS的表达来增加渗透压调节剂脯氨酸的合成。JERF 1还激活了阿坝生物合成关键酶基因OsABA 2和Os 03 g 0810800的表达，增加了水稻阿坝的合成。对JERF 1靶基因的顺式元件分析表明，其启动子中存在DRE/CRT和/或GCC盒，表明JERF 1可以通过与这些顺式元件结合来激活水稻中相关基因的表达。与其他ERF蛋白不同的是，JERF 1的构建性过表达并没有改变转基因水稻的生长和发育，这使得JEFR 1成为一个潜在的有用来源，在育种上更大的非生物胁迫耐受性。
Ethylene response factor (ERF) proteins regulate a variety of stress responses in plant. JERF1, a tomato ERF protein, can be induced by abscisic acid (ABA). Overexpression of JERF1 enhanced the tolerance of transgenic tobacco to high salt concentration, osmotic stress, and low temperature by regulating the expression of stress-responsive genes by binding to DRE/CRT and GCC-box cis-elements. In this research, we further report that overexpression of JERF1 significantly enhanced drought tolerance of transgenic rice. The overexpression activated the expression of stress-responsive genes and increased the synthesis of the osmolyte proline by regulating the expression of OsP5CS, encoding the proline biosynthesis key enzyme deltal-pyrroline-5-carboxylate synthetase. JERF1 also activated the expression of two ABA biosynthesis key enzyme genes, OsABA2 and Os03g0810800, and increased the synthesis of ABA in rice. Analysis of cis-elements of JERF1-targeted genes pointed to the existence of DRE/CRT and/or GCC box in their promoters, indicating that JERF1 could activate the expression of related genes in rice by binding to these cis-elements. Unlike some other ERF proteins, constructive overexpression of JERF1 did not change the growth and development of transgenic rice, which makes JEFR1 a potentially useful source in breeding for greater tolerance to abiotic stress.
登录
查看更多内容
影响因子：
7.4
作者：
Agrawal, GK;Yamazaki, M;Hirochika, H
通讯作者：
Hirochika, H
影响因子：
6.9
作者：
Andrew Peter Whitmore;W. Richard Whalley
通讯作者：
Andrew Peter Whitmore;W. Richard Whalley
影响因子：
3.5
作者：
K. Zuo;J. Qin;Jing-ya Zhao;H. Ling;Lida Zhang;Youfang Cao;K. Tang
通讯作者：
K. Zuo;J. Qin;Jing-ya Zhao;H. Ling;Lida Zhang;Youfang Cao;K. Tang
影响因子：
5.1
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
56.9
作者：
Schwartz, SH;Tan, BC;McCarty, DR
通讯作者：
McCarty, DR