Manipulation of pore structure during manufacture of agarose microspheres for bioseparation.
Manipulation of pore structure during manufacture of agarose microspheres for bioseparation.
复制标题
生物分离用琼脂糖微球制造过程中孔结构的调控
DOI：
10.1002/elsc.202000023
复制
发表时间：
2020-11
影响因子：
2.7
通讯作者：
Ma G
中科院分区：
文献类型：
--
作者：
Zhao L;Huang Y;Zhu K;Miao Z;Zhao J;Che XJ;Hao D;Zhang R;Ma G
通过改变琼脂糖类型和交联度，制备了具有可控孔结构的琼脂糖微球。不同的琼脂糖可以调整微球基质的凝胶形成，从而影响最终的孔隙结构。高分子量琼脂糖可以在微球中形成小孔隙，凝胶过滤色谱法测量的分布系数（Kav）值较低的填充柱证明了这一点。此外，较高的Kav值也表明，随着琼脂糖微球交联度的增加，形成的孔隙也更多、更大。无论是使用高分子量琼脂糖，还是提高交联度，最终都能提高填料柱的流动性能，从而提高分离效率。这为具有可控分离范围的高分辨率色谱奠定了基础，有利于下游工艺。
Agarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient (Kav) values measured by gel filtration chromatography. Further, higher Kav values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high‐resolution chromatography with a controllable separation range as beneficial for downstream process.
登录
查看更多内容
影响因子：
4
作者：
Ahearne, Mark;Kelly, Daniel J.
通讯作者：
Kelly, Daniel J.
DOI：
10.1016/s0021-9673(00)83800-5
发表时间：
1975-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
PORATH, J;LAAS, T;JANSON, JC
通讯作者：
JANSON, JC
影响因子：
11.2
作者：
Deszczynski, M;Kasapis, S;Mitchell, JR
通讯作者：
Mitchell, JR
影响因子：
8.6
作者：
Malik, D. J.;Webb, C.;Hoenich, N. A.
通讯作者：
Hoenich, N. A.
DOI：
10.1016/j.cep.2005.03.005
发表时间：
2005-10-01
影响因子：
4.3
作者：
Mu, Y;Lyddiatt, A;Pacek, AW
通讯作者：
Pacek, AW