Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.)
Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.)
复制标题
水稻早衰叶基因 psl1 的遗传分析和分子图谱 (Oryza sativa L.)
DOI：
10.1007/s11434-006-2222-x
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发表时间：
2006-12
期刊：
影响因子：
--
通讯作者：
Hu Jing
中科院分区：
文献类型：
--
作者：
Fang Yunxia;Xu Jiefen;Gu Minghong;Wang Jun;Wu Shujun;Liang Guohua;Zhou Yong;Zhou Lihui;Hu Jing
以粳稻品种中花11为材料，经60 Co-y辐射诱变，获得了水稻早老突变体psl 1（presencing leaf）。突变体叶片在达到最大长度后开始萎蔫，而野生型叶片可保持绿色25-35 d。本研究对鉴定出的突变体进行了遗传分析和基因定位。SSR标记分析表明，该突变体受位于第2染色体上的一对隐性基因（psl 1）控制。利用日本晴和93-11的序列差异，在psl 1锚定区附近开发了34个新的STS标记，对psl 1基因座进行了精细定位。psl 1进一步定位在STS标记STS 2 -19和STS 2 -26之间，遗传距离分别为0.43和0.11 cM，与STS 2 -25共分离。BAC重叠群被发现跨越psl 1基因座，该区域被限定为48 kb。这一结果为psl 1基因的克隆奠定了基础。
A rice psl1 (presenescing leaf) mutant was obtained from a japonica variety Zhonghua 11 via radiation of 60Co-y in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, while the leaves of the wild variety could keep green for 25–35 d. In this study, genetic analysis and gene mapping were carried out for the mutant identified. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (psl1) located on chromosome 2. Fine mapping of the psl1 locus was conducted with 34 new STS markers developed around psl1 anchored region based on the sequence diversity between Nipponbare and 93-11. The psl1 was further mapped between two STS markers, STS2-19 and STS2-26, with genetic distances of 0.43 and 0.11 cM, respectively, while cosegregated with STS2-25. A BAC contig was found to span the psl1 locus, the region being delimited to 48 kb. This result was very useful for cloning of the psl1 gene.
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影响因子：
7.4
作者：
BUCHANANWOLLASTON, V
通讯作者：
BUCHANANWOLLASTON, V
DOI：
10.1073/pnas.0931425100
发表时间：
2003-05-13
影响因子：
11.1
作者：
Lin, L;Liu, YG;Li, BJ
通讯作者：
Li, BJ
影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
5.1
作者：
Park, JH;Oh, SA;Nam, HG
通讯作者：
Nam, HG
影响因子：
64.8
作者：
Sasaki, T;Matsumoto, T;Gojohori, T
通讯作者：
Gojohori, T