Candida albicans as an Essential "Keystone" Component within Polymicrobial Oral Biofilm Models?
Candida albicans as an Essential "Keystone" Component within Polymicrobial Oral Biofilm Models?
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DOI：
10.3390/microorganisms9010059
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发表时间：
2020-12-28
期刊：
影响因子：
4.5
通讯作者：
Brown JL
中科院分区：
文献类型：
--
作者：
Young T;Alshanta OA;Kean R;Bradshaw D;Pratten J;Williams C;Woodall C;Ramage G;Brown JL
背景：现有的标准化生物膜分析主要集中在简单的单物种或细菌模型上。将白色念珠菌纳入复杂生物膜模型，可为口腔保健产品的开发提供更合适、更相关的多微生物生物膜。目的：本研究旨在评估有或没有白色念珠菌的多微生物口腔生物膜系统中王国间相互作用的重要性，并测试这些模型在体外对口腔治疗挑战的反应。材料和方法：在存在和不存在白色念珠菌的情况下，平行创建多微生物生物膜（分别含有5种和10种细菌），并使用临床相关的抗菌剂进行挑战。分别用蓝靛素染色法和结晶紫染色法测定了这些复合生物膜的代谢谱和生物量。利用定量PCR技术评估微生物负荷的组成变化。额外的测定，用于测量pH值和乳酸，包括监测毒力“生物标志物”的波动。结果：观察到白色念珠菌存在时代谢活性和生物量水平升高。在白色念珠菌的存在下，细菌负荷增加了10倍以上。实验显示白色念珠菌的加入影响了生物膜的毒力谱。白色念珠菌不影响生物膜对不同处理的短期孵育反应。结论：本文描述的界间生物膜结构坚固，并表现出可复制模型的所有特征。据我们所知，这些数据首次验证了酵母可能作为口腔生物膜潜在“基石”成分的假设。
Background: Existing standardized biofilm assays focus on simple mono-species or bacterial-only models. Incorporating Candida albicans into complex biofilm models can offer a more appropriate and relevant polymicrobial biofilm for the development of oral health products. Aims: This study aimed to assess the importance of interkingdom interactions in polymicrobial oral biofilm systems with or without C. albicans, and test how these models respond to oral therapeutic challenges in vitro. Materials and Methods: Polymicrobial biofilms (two models containing 5 and 10 bacterial species, respectively) were created in parallel in the presence and absence of C. albicans and challenged using clinically relevant antimicrobials. The metabolic profiles and biomasses of these complex biofilms were estimated using resazurin dye and crystal violet stain, respectively. Quantitative PCR was utilized to assess compositional changes in microbial load. Additional assays, for measurements of pH and lactate, were included to monitor fluctuations in virulence “biomarkers.” Results: An increased level of metabolic activity and biomass in the presence of C. albicans was observed. Bacterial load was increased by more than a factor of 10 in the presence of C. albicans. Assays showed inclusion of C. albicans impacted the biofilm virulence profiles. C. albicans did not affect the biofilms’ responses to the short-term incubations with different treatments. Conclusions: The interkingdom biofilms described herein are structurally robust and exhibit all the hallmarks of a reproducible model. To our knowledge, these data are the first to test the hypothesis that yeasts may act as potential “keystone” components of oral biofilms.
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影响因子：
3.1
作者：
Falsetta, Megan L.;Klein, Marlise I.;Koo, Hyun
通讯作者：
Koo, Hyun
影响因子：
6.8
作者：
Delaney, Christopher;O'Donnell, Lindsay E.;Ramage, Gordon
通讯作者：
Ramage, Gordon
影响因子：
4.5
作者：
Arias, Lais Salomao;Brown, Jason L.;Ramage, Gordon
通讯作者：
Ramage, Gordon
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
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作者：
通讯作者：
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影响因子：
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作者：
Baliga S;Muglikar S;Kale R
通讯作者：
Kale R