A review of microdialysis coupled to microchip electrophoresis for monitoring biological events.
A review of microdialysis coupled to microchip electrophoresis for monitoring biological events.
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DOI：
10.1016/j.chroma.2014.12.086
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发表时间：
2015-02-20
期刊：
影响因子：
--
通讯作者：
Lunte SM
中科院分区：
文献类型：
--
作者：
Saylor RA;Lunte SM
微透析是一种功能强大的采样技术，能够监测体外和体内的动态过程。微透析与色谱或电泳方法的组合产生沿着选择性检测方法产生能够在接近真实的时间内监测多种分析物的“基于分离的传感器”。微透析样品的分析需要快速（<1分钟）、低体积要求（nL-pL）的技术，并且理想情况下可以在线使用。微芯片电泳满足这些要求，也允许集成样品制备和操作与检测策略直接在芯片上的可能性。微透析结合微芯片电泳已被用于监测生物事件在体内和体外。本文综述了耦合微透析采样和微芯片电泳的技术考虑，包括各种接口设计，以及目前在该领域的应用。
Microdialysis is a powerful sampling technique that enables monitoring of dynamic processes in vitro and in vivo. The combination of microdialysis with chromatographic or electrophoretic methods yields along with selective detection methods yields a “separation-based sensor” capable of monitoring multiple analytes in near real time. Analysis of microdialysis samples requires techniques that are fast (<1 min), have low volume requirements (nL–pL), and, ideally, can be employed on-line. Microchip electrophoresis fulfills these requirements and also permits the possibility of integrating sample preparation and manipulation with detection strategies directly on-chip. Microdialysis coupled to microchip electrophoresis has been employed for monitoring biological events in vivo and in vitro. This review discusses technical considerations for coupling microdialysis sampling and microchip electrophoresis, including various interface designs, and current applications in the field.
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影响因子：
6.1
作者：
Carroll, Susan;Crain, Mark M.;Baldwin, Richard P.
通讯作者：
Baldwin, Richard P.
影响因子：
7.4
作者：
Allen PB;Chiu DT
通讯作者：
Chiu DT
影响因子：
7.4
作者：
Culbertson, CT;Tugnawat, Y;Gonda, SR
通讯作者：
Gonda, SR
影响因子：
3.7
作者：
AULT, JM;RILEY, CM;LUNTE, CE
通讯作者：
LUNTE, CE
DOI：
10.1016/s0731-7085(98)00182-4
发表时间：
1999-03-01
影响因子：
3.4
作者：
Chaurasia, CS;Chen, CE;Ashby, CR
通讯作者：
Ashby, CR