Catalase Enhances Growth and Biofilm Production of Mycoplasma pneumoniae.

Catalase Enhances Growth and Biofilm Production of Mycoplasma pneumoniae.
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DOI:
10.1007/s00284-015-0822-x
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发表时间:
2015-08
影响因子:
2.6
通讯作者:
Dybvig, Kevin
Dybvig, Kevin
中科院分区:
生物学4区
文献类型:
--
作者:
Simmons, Warren L.;Dybvig, Kevin

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肺炎支原体导致人类慢性呼吸道疾病。被认为对定殖重要的因素包括支原体在上皮表面上形成生物膜的能力和产生过氧化氢以损伤宿主组织。几乎所有的支原体,包括M. pneumoniae,缺乏超氧化物歧化酶和过氧化氢酶,并且在过氧化物产生和生长之间应该存在平衡。我们在这里表明,向培养物中添加过氧化氢酶可以增强生物膜的形成并改变结构。琼脂中加入过氧化氢酶可增加检测到的菌落形成单位的数量,因此可提高支原体疾病的临床诊断。
Mycoplasma pneumoniae causes chronic respiratory disease in humans. Factors thought to be important for colonization include the ability of the mycoplasma to form a biofilm on epithelial surfaces and the production of hydrogen peroxide to damage host tissue. Almost all of the mycoplasmas, including M. pneumoniae, lack superoxide dismutase and catalase and a balance should exist between peroxide production and growth. We show here that the addition of catalase to cultures enhanced the formation of biofilms and altered the structure. The incorporation of catalase in agar increased the number of colony-forming units detected and hence could improve the clinical diagnosis of mycoplasmal diseases.
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