Ca(2+)-supplying black phosphorus-based scaffolds fabricated with microfluidic technology for osteogenesis.
Ca(2+)-supplying black phosphorus-based scaffolds fabricated with microfluidic technology for osteogenesis.
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DOI：
10.1016/j.bioactmat.2021.04.014
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发表时间：
2021-11
影响因子：
18.9
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li Z;Zhang X;Ouyang J;Chu D;Han F;Shi L;Liu R;Guo Z;Gu GX;Tao W;Jin L;Li J
有效的成骨仍然是治疗骨缺损的一个挑战。人造骨支架的出现提供了一个有吸引力的解决方案。在这项工作中，提出了一种新的生物矿化策略，通过维持磷（P）、钙（Ca）和硅（Si）等营养物质的供应来促进成骨。我们通过微流控技术开发了基于黑磷（BP）的三维纳米复合纤维支架，为骨缺损治疗提供丰富的必需离子。纤维支架由 3D 聚（L-乳酸）（PLLA）纳米纤维（3D NF）、BP 纳米片和羟基磷灰石（HA）多孔 SiO2 纳米颗粒制成。 3D BP@HA NF具有三个优点：i）稳定连接的孔允许骨髓间充质干细胞（BMSC）轻松进入3D纤维支架内部，进行骨修复和成骨； ii) NFs中丰富的营养物质强烈改善骨修复区域的成骨分化； iii)纤维支架的光热效应促进骨形成所需元素的释放，从而实现加速成骨。体外和体内结果均表明，3D BP@HA NFs在近红外激光的辅助下，在促进骨再生方面表现出良好的性能。此外，微流控技术使得以低成本、快速加工、高通量和大规模生产获得高质量的3D BP@HA NFs成为可能，大大改善了临床应用前景。这也是第一个基于BP的可自供Ca2+的骨支架平台，这对于患有骨缺损的老年患者或因钙流失而导致骨骼受损的患者来说可能是福音。提出了一种新的生物矿化策略来促进成骨。通过微流控技术构建了基于 BP 的 3D 纳米复合纤维支架。形成的纤维支架可以维持包括P、Ca和Si在内的营养物质的供应。
Effective osteogenesis remains a challenge in the treatment of bone defects. The emergence of artificial bone scaffolds provides an attractive solution. In this work, a new biomineralization strategy is proposed to facilitate osteogenesis through sustaining supply of nutrients including phosphorus (P), calcium (Ca), and silicon (Si). We developed black phosphorus (BP)-based, three-dimensional nanocomposite fibrous scaffolds via microfluidic technology to provide a wealth of essential ions for bone defect treatment. The fibrous scaffolds were fabricated from 3D poly (l-lactic acid) (PLLA) nanofibers (3D NFs), BP nanosheets, and hydroxyapatite (HA)-porous SiO2 nanoparticles. The 3D BP@HA NFs possess three advantages: i) stably connected pores allow the easy entrance of bone marrow-derived mesenchymal stem cells (BMSCs) into the interior of the 3D fibrous scaffolds for bone repair and osteogenesis; ii) plentiful nutrients in the NFs strongly improve osteogenic differentiation in the bone repair area; iii) the photothermal effect of fibrous scaffolds promotes the release of elements necessary for bone formation, thus achieving accelerated osteogenesis. Both in vitro and in vivo results demonstrated that the 3D BP@HA NFs, with the assistance of NIR laser, exhibited good performance in promoting bone regeneration. Furthermore, microfluidic technology makes it possible to obtain high-quality 3D BP@HA NFs with low costs, rapid processing, high throughput and mass production, greatly improving the prospects for clinical application. This is also the first BP-based bone scaffold platform that can self-supply Ca2+, which may be the blessedness for older patients with bone defects or patients with damaged bones as a result of calcium loss. A new biomineralization strategy is proposed to facilitate osteogenesis. A BP-based 3D nanocomposite fibrous scaffold was constructed via microfluidic technology. The formed fibrous scaffold can sustain supply of nutrients including P, Ca, and Si.
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