Novel microRNAs differentially expressed during aging in the mouse brain.
Novel microRNAs differentially expressed during aging in the mouse brain.
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DOI：
10.1371/journal.pone.0040028
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Slack F
中科院分区：
文献类型：
--
作者：
Inukai S;de Lencastre A;Turner M;Slack F
microRNA（miRNAs）是一种内源性小RNA分子，在转录后调节基因表达。在秀丽隐杆线虫中的工作已经表明，特定的miRNA在寿命调节和各种年龄相关的途径中起作用，但是miRNA在脊椎动物衰老中的作用还不清楚。我们通过深度测序检测了年轻和老年小鼠全脑中小RNA的表达，并在此报告了558种已知miRNA的表达和41种新miRNA的鉴定。在这些miRNA中，75种已知的和18种新的miRNA表现出大于2.0倍的表达变化。在我们的研究中，大多数表达的miRNA在老年大脑中的相对丰度下降，与在衰老组织和生物体中的其他miRNA研究中观察到的趋势一致。靶点预测分析表明，我们的许多新的衰老相关的miRNA靶向胰岛素信号通路中的基因，这是衰老相关遗传网络的中心节点。因此，这些新的miRNAs可以调节大脑中与衰老相关的功能。由于许多小鼠miRNAs在人类中是保守的，我们在这里报告的衰老影响的大脑miRNAs可能代表了在人类大脑衰老过程中也起作用的新的调控基因。
MicroRNAs (miRNAs) are endogenous small RNA molecules that regulate gene expression post-transcriptionally. Work in Caenorhabditis elegans has shown that specific miRNAs function in lifespan regulation and in a variety of age-associated pathways, but the roles of miRNAs in the aging of vertebrates are not well understood. We examined the expression of small RNAs in whole brains of young and old mice by deep sequencing and report here on the expression of 558 known miRNAs and identification of 41 novel miRNAs. Of these miRNAs, 75 known and 18 novel miRNAs exhibit greater than 2.0-fold expression changes. The majority of expressed miRNAs in our study decline in relative abundance in the aged brain, in agreement with trends observed in other miRNA studies in aging tissues and organisms. Target prediction analysis suggests that many of our novel aging-associated miRNAs target genes in the insulin signaling pathway, a central node of aging-associated genetic networks. These novel miRNAs may thereby regulate aging-related functions in the brain. Since many mouse miRNAs are conserved in humans, the aging-affected brain miRNAs we report here may represent novel regulatory genes that also function during aging in the human brain.
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