Resolving Power and Collision Cross Section Measurement Accuracy of a Prototype High-Resolution Ion Mobility Platform Incorporating Structures for Lossless Ion Manipulation.
Resolving Power and Collision Cross Section Measurement Accuracy of a Prototype High-Resolution Ion Mobility Platform Incorporating Structures for Lossless Ion Manipulation.
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DOI：
10.1021/jasms.1c00056
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发表时间：
2021-04-07
影响因子：
3.2
通讯作者：
McLean, John A.
中科院分区：
文献类型：
--
作者：
May, Jody C.;Leaptrot, Katrina L.;Rose, Bailey S.;Moser, Kelly L. Wormwood;Deng, Liulin;Maxon, Laura;DeBord, Daniel;McLean, John A.
描述了一种与商用高分辨率质谱计（MS）接口的无损离子操纵离子迁移（SLIM IM）平台的生产原型结构。SLIM IM实现了跨~ 13m路径长度的行波离子迁移技术，用于高分辨率IM （hrm）分离。对SLIM IM平台的分辨率（CCS/ΔCCS）进行了各种参数（行波速度、振幅和波形）的基准测试，结果表明，在广泛的质量范围内，可以获得超过200的分辨率。在一些情况下，分辨率达到了300以上，特别是在离子从非选择性“冲浪”运动转变为流动性选择性离子漂移的波浪条件下，这对应于离子速度约为行波速度的30-70%。对一系列不能单独用MS分离的异构体和异构化合物进行了分离能力评估，包括反序列肽（SDGRG和GRGDS），甘油三酯双键位置异构体（TG 3,6,9和TG 6,9,12），三糖（麦芽糖糖，棉子糖，异麦芽糖三糖和麦芽糖三糖）和神经节苷脂（GD1b和GD1a）。SLIM IM平台可以分辨出相应的同分异构体混合物，这是用漂管仪器的标准分辨率无法分辨的（~ 50）。一般来说，SLIM IM-MS平台能够分辨出低至0.6%的分离峰，而不需要针对特定的分离窗口或漂移时间。在高分辨率条件下，CCS测量偏差<0.5%。重要的是，所有被调查的分析物都能够获得高分辨率条件（bbb200），这表明该仪器非常适合宽带hrm分离，这在全球非目标应用中很重要。
A production prototype structures for lossless ion manipulation ion mobility (SLIM IM) platform interfaced to a commercial high-resolution mass spectrometer (MS) is described. The SLIM IM implements the traveling wave ion mobility technique across a ∼13m path length for high-resolution IM (HRIM) separations. The resolving power (CCS/ΔCCS) of the SLIM IM stage was benchmarked across various parameters (traveling wave speeds, amplitudes, and waveforms), and results indicated that resolving powers in excess of 200 can be accessed for a broad range of masses. For several cases, resolving powers greater than 300 were achieved, notably under wave conditions where ions transition from a nonselective “surfing” motion to a mobility-selective ion drift, that corresponded to ion speeds approximately 30–70% of the traveling wave speed. The separation capabilities were evaluated on a series of isomeric and isobaric compounds that cannot be resolved by MS alone, including reversed-sequence peptides (SDGRG and GRGDS), triglyceride double-bond positional isomers (TG 3, 6, 9 and TG 6, 9, 12), trisaccharides (melezitose, raffinose, isomaltotriose, and maltotriose), and ganglioside lipids (GD1b and GD1a). The SLIM IM platform resolved the corresponding isomeric mixtures, which were unresolvable using the standard resolution of a drift-tube instrument (∼50). In general, the SLIM IM-MS platform is capable of resolving peaks separated by as little as ∼0.6% without the need to target a specific separation window or drift time. Low CCS measurement biases <0.5% were obtained under high resolving power conditions. Importantly, all the analytes surveyed are able to access high-resolution conditions (>200), demonstrating that this instrument is well-suited for broadband HRIM separations important in global untargeted applications.
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影响因子：
7.4
作者：
Chen TC;Ibrahim YM;Webb IK;Garimella SV;Zhang X;Hamid AM;Deng L;Karnesky WE;Prost SA;Sandoval JA;Norheim RV;Anderson GA;Tolmachev AV;Baker ES;Smith RD
通讯作者：
Smith RD
DOI：
10.1007/s13361-014-0976-y
发表时间：
2014-11
影响因子：
3.2
作者：
Garimella, Sandilya V. B.;Ibrahim, Yehia M.;Webb, Ian K.;Tolmachev, Aleksey V.;Zhang, Xinyu;Prost, Spencer A.;Anderson, Gordon A.;Smith, Richard D.
通讯作者：
Smith, Richard D.
DOI：
10.1007/s13361-017-1680-5
发表时间：
2017-07
影响因子：
3.2
作者：
Garimella SVB;Webb IK;Prabhakaran A;Attah IK;Ibrahim YM;Smith RD
通讯作者：
Smith RD
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
DOI：
10.1007/s12127-016-0198-z
发表时间：
2016-09-01
影响因子：
--
作者：
Adams, Kendra J.;Montero, Dennise;Fernandez-Lima, Francisco
通讯作者：
Fernandez-Lima, Francisco