R gene triplication confers European fodder turnip with improved clubroot resistance.
R gene triplication confers European fodder turnip with improved clubroot resistance.
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R基因三倍体赋予欧洲饲料萝卜改善根肿病抗性
DOI：
10.1111/pbi.13827
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发表时间：
2022-08
影响因子：
13.8
通讯作者：
Zhang, Chunyu
中科院分区：
文献类型：
--
作者：
Yang, Zhiquan;Jiang, Yingfen;Gong, Jianfang;Li, Qian;Dun, Bicheng;Liu, Dongxu;Yin, Feifan;Yuan, Lei;Zhou, Xueqing;Wang, Huiying;Wang, Jing;Zhan, Zongxiang;Shah, Nadil;Nwafor, Chinedu Charles;Zhou, Yuanwei;Chen, Peng;Zhu, Li;Li, Shisheng;Wang, Bingrui;Xiang, Jun;Zhou, Yongming;Li, Zaiyun;Piao, Zhongyun;Yang, Qingyong;Zhang, Chunyu
根腐病是世界上许多重要十字花科蔬菜和油料作物的主要病害之一。目前，油菜品种中已鉴定出不同的棍棒病抗性位点，这给比较和利用这些位点带来了困难。欧洲饲料萝卜ECD04被认为是最有价值的CR育种资源之一。为了探索ECD04中CR的遗传和进化基础，我们对ECD04基因组进行了从头组装测序，鉴定出978个候选R基因。随后，将28个已发表的CR基因座物理定位到ECD04基因组中的15个基因座，其中包括62个候选CR基因。其中，CRA3.7.1和CRA8.2.4两个CR基因进行了功能验证。系统发育分析表明，CRA3.7.1和CRA8.2.4在全基因组三倍复制（WGT）事件发生前起源于同一个祖先。在球茎病易感的芸苔品种中，CR基因同源物受到转座因子（TE）插入的影响，导致CR功能丧失。结果表明，油菜中现有的功能性CR基因和其他芸苔属植物的非功能性CR基因均来源于WGT之前的一个共同祖先基因。最后，提出了CR基因进化的假设，以供进一步探讨。
Summary Clubroot is one of the most important diseases for many important cruciferous vegetables and oilseed crops worldwide. Different clubroot resistance (CR) loci have been identified from only limited species in Brassica, making it difficult to compare and utilize these loci. European fodder turnip ECD04 is considered one of the most valuable resources for CR breeding. To explore the genetic and evolutionary basis of CR in ECD04, we sequenced the genome of ECD04 using de novo assembly and identified 978 candidate R genes. Subsequently, the 28 published CR loci were physically mapped to 15 loci in the ECD04 genome, including 62 candidate CR genes. Among them, two CR genes, CRA3.7.1 and CRA8.2.4, were functionally validated. Phylogenetic analysis revealed that CRA3.7.1 and CRA8.2.4 originated from a common ancestor before the whole‐genome triplication (WGT) event. In clubroot susceptible Brassica species, CR‐gene homologues were affected by transposable element (TE) insertion, resulting in the loss of CR function. It can be concluded that the current functional CR genes in Brassica rapa and non‐functional CR genes in other Brassica species were derived from a common ancestral gene before WGT. Finally, a hypothesis for CR gene evolution is proposed for further discussion.
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影响因子：
5.6
作者：
Fredua-Agyeman, Rudolph;Yu, Zhiyu;Strelkov, Stephen E.
通讯作者：
Strelkov, Stephen E.
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影响因子：
56.9
作者：
Deng, Yiwen;Zhai, Keran;He, Zuhua
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He, Zuhua
影响因子：
9.3
作者：
Durand NC;Shamim MS;Machol I;Rao SS;Huntley MH;Lander ES;Aiden EL
通讯作者：
Aiden EL
影响因子：
16
作者：
Grob, Stefan;Schmid, Marc W.;Grossniklaus, Ueli
通讯作者：
Grossniklaus, Ueli
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A