Species misidentification in local markets: Discrepancies between reporting and molecular identification of bushmeat species in northern Uganda.
Species misidentification in local markets: Discrepancies between reporting and molecular identification of bushmeat species in northern Uganda.
复制标题
DOI：
10.1016/j.onehlt.2021.100251
复制
发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Souza M
中科院分区：
文献类型：
--
作者：
Dell B;Masembe C;Gerhold R;Willcox A;Okafor C;Souza M
狩猎和消费丛林肉虽然在许多地区被广泛用作家庭收入和营养的必要补充，但对公共卫生和野生动物保护工作构成威胁。在北方乌干达，蝙蝠和灵长类动物的消费，两个野生动物群体往往牵连到人畜共患病的出现，并没有被广泛的文化接受;然而，猎人报告说，这些物种经常被狩猎和出售的文化理想的物种，如羚羊和野猪。为了调查市场上肉类误认的流行情况，我们从默奇森福尔斯国家公园附近的23个社区收集了229份肉类样本。在采集每份样品时记录报告的菌种。进行靶向哺乳动物cyt B和12 s rRNA基因的PCR和测序，以使用NCBI BLAST将样品鉴定为最低分类单位。总体而言，27.9%（61/219）的样本在报告的种属和BLAST分析之间具有不同的结果。共鉴定出34种，最常见的野生动物是水羚（31.5%）、野猪（13.7%）和黑鼠（5.9%）。这些数据揭示了乌干达北方的肉类消费者的公共健康风险，因为他们在购买肉类时无法评估与物种相关的风险，也无法采取适当的预防措施防止人畜共患病病原体的暴露。这些数据还提供了深入了解当地社区成员的区域猎人猎物偏好和市场偏好，这可能会为该地区的保护战略提供信息。最常见的野生动物物种是羚羊。销售点报告的种属与PCR确认的种属之间存在近30%的差异。从猎人的样本有统计学上的差异低于厨师和经销商。
Bushmeat hunting and consumption, although widely utilized as necessary supplement to household income and nutrition in many regions, presents threats to public health and wildlife conservation efforts. In northern Uganda, consumption of bats and primates, two wildlife groups often implicated in zoonotic disease emergence, is not widely culturally accepted; however, these species are reported by hunters to often be hunted and sold as culturally desirable species, like antelope and warthog. To investigate the prevalence of market bushmeat misidentifiction, we collected 229 bushmeat samples from 23 communities adjacent to Murchison Falls National Park. Reported species was recorded on acquisition for each sample. PCR targeting mammalian cyt b and 12 s rRNA genes and sequencing were performed to identify samples to the lowest taxonomic unit using NCBI BLAST. Overall, 27.9% (61/219) of samples had disparate results between species reported and BLAST analysis. Thirty-four species were identified, with the most frequent wildlife being waterbuck (31.5%), warthog (13.7%), and black rat (5.9%). These data reveal a public health risk for bushmeat consumers in northern Uganda as they cannot assess species-related risk when purchasing bushmeat and take appropriate precautions against zoonotic pathogen exposure. These data also provide insight into regional hunter prey preference and market preference of local community members which may inform conservation strategy in the region. The most frequently identified wildlife species were antelopes. Nearly a 30% discrepancy between species reported at point of sale and PCR confirmation. Samples from hunters had statistically lower discrepancy than cooks and dealers.
登录
查看更多内容
影响因子：
6.3
作者：
Fa, John E.;Olivero, Jesus;Vargas, Juan M.
通讯作者：
Vargas, Juan M.
影响因子：
2.7
作者：
Fa, JE;Currie, D;Meeuwig, J
通讯作者：
Meeuwig, J
影响因子：
3.7
作者：
Dell BM;Souza MJ;Willcox AS
通讯作者：
Willcox AS
影响因子：
64.8
作者：
Jones KE;Patel NG;Levy MA;Storeygard A;Balk D;Gittleman JL;Daszak P
通讯作者：
Daszak P
DOI：
10.1038/nrmicro.2017.45
发表时间：
2017-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Plowright RK;Parrish CR;McCallum H;Hudson PJ;Ko AI;Graham AL;Lloyd-Smith JO
通讯作者：
Lloyd-Smith JO