Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle
Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle
复制标题
miRNA 和 mRNA 的综合分析揭示了奶牛脂质代谢的调节
DOI：
10.1007/s10142-021-00786-9
复制
发表时间：
2021-05
影响因子：
2.9
通讯作者：
Runjun Yang
中科院分区：
文献类型：
--
作者：
Lixin Xia;Zhihui Zhao;Xianzhong Yu;Chunyan Lu;Ping Jiang;Haibin Yu;Xiaohui Li;Xiang Yu;Juan Liu;Xibi Fang;Runjun Yang
奶牛乳腺上皮细胞的脂质代谢一直是奶牛乳脂率研究的重点。功能性microRNA可以通过调节候选基因的表达来影响脂质代谢。本研究的目的是筛选和鉴定与乳脂代谢相关的差异表达的miRNAs、候选基因和共调控途径。为了实现这一目标，我们使用了来自泌乳中期高乳脂率（H，4.85%）和低乳脂率（L，3.41%）奶牛乳腺上皮细胞的miRNA和转录组数据。190个差异表达基因和33个差异表达的miRNAs在相关调控网络中显著富集，其中27个候选基因由18个差异表达的miRNAs调控，显著富集在脂代谢相关通路中（p<0. 05）。采用荧光素酶报告基因分析和定量RT-PCR验证PDE 4D和bta-miR-148 a、PEG 10和bta-miR-877、SOD 3和bta-miR-2382- 5 p以及ADAMTS 1和bta-miR-2425- 5 p之间的靶点关系。BMEC中甘油三酯生成的检测显示，bta-miR-21- 3 p和bta-miR-148 a促进甘油三酯合成，而bta-miR-124 a、bta-miR-877、bta-miR-2382- 5 p和bta-miR-2425- 5 p抑制甘油三酯合成。联合分析可在奶牛乳腺系统共表达网络中发现参与脂肪代谢的功能性miRNAs和调控候选基因，有助于了解乳脂代谢相关遗传元件和基因信号网络的潜在调控机制。
Lipid metabolism in bovine mammary epithelial cells has been the primary focus of the research of milk fat percentage of dairy cattle. Functional microRNAs can affect lipid metabolism by regulating the expression of candidate genes. The purpose of the study was to screen and identify differentially expressed miRNAs, candidate genes, and co-regulatory pathways related to the metabolism of milk fat. To achieve this aim, we used miRNA and transcriptome data from the mammary epithelial cells of dairy cattle with high (H, 4.85%) and low milk fat percentages (L, 3.41%) during mid-lactation. One hundred ninety differentially expressed genes and 33 differentially expressed miRNAs were significantly enriched in related regulatory networks, of which 27 candidate genes regulated by 18 differentially expressed miRNAs significantly enriched in pathways related to lipid metabolism (p< 0.05). Target relationships betweenPDE4Dand bta-miR-148a,PEG10and bta-miR-877,SOD3and bta-miR-2382-5p, andADAMTS1and bta-miR-2425-5p were verified using luciferase reporter assays and quantitative RT-PCR. The detection of triglyceride production in BMECs showed that bta-miR-21-3p and bta-miR-148a promote triglyceride synthesis, whereas bta-miR-124a, bta-miR-877, bta-miR-2382-5p, and bta-miR-2425-5p inhibit triglyceride synthesis. The conjoint analysis could identify functional miRNAs and regulatory candidate genes involved in lipid metabolism within the co-expression networks of the dairy cattle mammary system, which contributes to the understanding of potential regulatory mechanisms of genetic element and gene signaling networks involved in milk fat metabolism.
登录
查看更多内容
影响因子：
4.4
作者：
Li Z;Liu H;Jin X;Lo L;Liu J
通讯作者：
Liu J
系统的MicroRNAOME分析揭示了microRNAS在牛奶蛋白代谢和质量中的作用：低质量饲料利用的见解。
影响因子：
4.6
作者：
Wang D;Liang G;Wang B;Sun H;Liu J;Guan le L
通讯作者：
Guan le L
影响因子：
2.4
作者：
Yang J;Jiang J;Liu X;Wang H;Guo G;Zhang Q;Jiang L
通讯作者：
Jiang L
MicroRNA-106b 通过 ATP 结合盒亚家族 A 成员 1 (ABCA1) 调节牛乳腺上皮细胞中的乳脂代谢
影响因子：
6.1
作者：
Chen, Zhi;Chu, Shuangfeng;Yang, Zhangping
通讯作者：
Yang, Zhangping
影响因子：
168.9
作者：
D. Rutstein;W. Castelli;R. Nickerson
通讯作者：
D. Rutstein;W. Castelli;R. Nickerson