Morphological plasticity of motor axons in Drosophila mutants with altered excitability
Morphological plasticity of motor axons in Drosophila mutants with altered excitability
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兴奋性改变的果蝇突变体运动轴突的形态可塑性
DOI：
10.1523/jneurosci.10-11-03754.1990
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发表时间：
1990
期刊：
影响因子：
4
通讯作者：
Chun
中科院分区：
文献类型：
--
作者：
Vivian Budnik;Yi Zhong;Chun
对果蝇突变体进行了解剖和电生理研究，以确定改变的电活动对幼虫神经肌肉连接的发育和维持的影响。我们检测了(1)超兴奋突变体Shaker （Sh）、ether a go-go （eag）、Hyperkinetic （Hk）和Duplication of para+ (Dp para+)的运动轴突末端；(2)兴奋性降低，无动作电位（napts）和麻痹（part 1）的突变体。利用特异性染色昆虫神经元的抗hrp免疫细胞化学方法，观察了支配幼虫体壁肌肉的神经末梢。野生型幼虫运动轴突终末呈定型分布。然而，在eag和Sh等位基因的组合中，神经支配的基本模式发生了改变。肌肉上的高阶轴突分支数量和神经突上的静脉曲张数量都有所增加。在双突变体Hk eag中发现了类似的现象，在Dp para+和Dp para+ Sh突变体中也发现了类似的现象，但程度较轻。众所周知，在允许的温度下，napts（而不是第1部分）突变降低了幼虫运动轴突的兴奋性，抑制了Sh、eg和Hk的行为表型。在突变体中（在允许温度下饲养），观察到分支的程度略有下降。然而，当与egsh结合时，napts完全逆转了egsh突变体的形态异常。在第1部分和Sh突变体中没有观察到这种逆转。在半完整的幼虫制备中，通过细胞外记录分析了神经肌肉连接处的内源性电活动模式。野生型体壁肌肉的记录显示出有节奏的刺突爆发。在egsh突变体中，这种节奏性活动伴随着或叠加在强烈的强音活动周期上。这种异常的活动模式可以部分地被napts和egsh联合抑制。
An anatomical and electrophysiological study of Drosophila mutants has been made to determine the effect of altered electrical activity on the development and maintenance of larval neuromuscular junctions. We examined motor axon terminals of (1) hyperexcitable mutants Shaker (Sh), ether a go-go (eag), Hyperkinetic (Hk), and Duplication of para+ (Dp para+); and (2) mutants with reduced excitability, no action potential (napts) and paralytic (parats 1). Nerve terminals innervating larval body-wall muscles were visualized by using anti-HRP immunocytochemistry, which specifically stains neurons in insect species. In wild-type larvae, motor axon terminals were distributed in a stereotypic fashion. However, in combinations of eag and Sh alleles, the basic pattern of innervation was altered. There was an increase in both the number of higher-order axonal branches over the muscles and the number of varicosities on the neurites. A similar phenomenon was found in the double mutant Hk eag and, to a lesser extent, in Dp para+ and Dp para+ Sh mutants. It is known that at permissive temperature the napts, but not parats 1, mutation decreases excitability of larval motor axons and suppresses the behavioral phenotypes of Sh, eag, and Hk. In the mutant napts (reared at permissive temperature), a slight decrease in the extent of branching was observed. Yet, when combined with eag Sh, napts completely reversed the morphological abnormality in eag Sh mutants. No such reversion was observed in parats 1 eag Sh mutants. The endogenous patterns of electrical activity at the neuromuscular junction were analyzed by extracellular recordings in a semi-intact larval preparation. Recordings from wild-type body-wall muscles revealed rhythmic bursts of spikes. In eag Sh mutants, this rhythmic activity was accompanied by or superimposed on periods of strong tonic activity. This abnormal pattern of activity could be partially suppressed by napts in combination with eag Sh.
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果蝇钠通道基因对行为和轴突兴奋性的剂量影响。
影响因子：
3.3
作者：
Stern,M;Kreber,R;Ganetzky,B
通讯作者：
Ganetzky,B
苍蝇感光突触：它们的发育、进化和可塑性。
DOI：
10.1002/neu.480200503
发表时间：
1989
期刊：
Journal of neurobiology
影响因子：
--
作者：
Meinertzhagen,IA
通讯作者：
Meinertzhagen,IA
影响因子：
56.9
作者：
COHAN, CS;KATER, SB
通讯作者：
KATER, SB
竞争控制已确定的轴突树枝化的生长。
DOI：
10.1126/science.6729457
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Murphey,RK;Lemere,CA
通讯作者：
Lemere,CA
影响因子：
56.9
作者：
WU, CF;GANETZKY, B;LIU, AX
通讯作者：
LIU, AX