Expression and effects of miR-137 in patients with erectile dysfunction
Expression and effects of miR-137 in patients with erectile dysfunction
复制标题
勃起功能障碍患者中miR-137的表达及影响
DOI：
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Wenrui Wang
中科院分区：
文献类型：
--
作者：
Yaochun Fan;Ruichun Fan;Xiong Lai;Xiaoling Tian;Sheng Gao;Nan Hu;Xiaoyan Zhang;Shaohong Yan;Yuhan Qin;Wanjin Xing;Wenrui Wang
目的：分析老年勃起功能障碍患者miR -137的表达情况。（ED）和年轻健康的志愿者。方法：对阴茎海绵体的形态和血流进行观察。所有患者均通过血管造影进行分析。采用实时荧光RT-PCR检测miR-137的表达。在取自外周血的干细胞样本中。Western blot检测CDK16的表达。miR-137的靶蛋白。结果：miR-137在老年ED患者中的表达明显高于年轻ED患者。与IIEF-5得分呈负相关。CDK16蛋白在pros-中表达较低。ED患者的分泌物样本含量高于健康对照组。健康志愿者的平均阴茎血流量为98.83±1.32 mL/min，老年ED患者的平均阴茎血流量为31.28±1.75 mL/min。有显著差异。两组间临床症状差异较大。结论：ED的程度与患者之间存在密切的关系。miR-137的表达，这可能抑制CDK16的生理表达。
Objective: We analyzed the expression of microRNA (miR)-137 in elderly patients with erectile dysfunction .(ED) and young healthy volunteers. Methods: The morphology and blood flow of the penile corpus cavernosum of .all patients were analyzed via angiography. The miR-137 expression was detected by real-time fluorescent RT-PCR .in stem cell samples taken from peripheral blood. Western blot was used to detect the expression of CDK16, the .target protein of miR-137. Results: miR-137 expression was significantly higher in elderly ED patients than in young .healthy volunteers, and were negatively correlated with IIEF-5 scores. CDK16 protein expression was lower in pros-.tate secretion samples of ED patients than in healthy controls. The average rate of penile blood flow was 98.83 ± .1.32 mL/min in healthy volunteers and 31.28 ± 1.75 mL/min in elderly ED patients. There were significant differ-.ences in clinical signs between these groups. Conclusion: There was a close correlation between the degree of ED .and miR-137 expression, which may inhibit the physiological expression of CDK16.
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DOI：
10.1006/bbrc.2001.5271
发表时间：
2001-07-27
影响因子：
3.1
作者：
Corbeil, D;Fargeas, CA;Huttner, WB
通讯作者：
Huttner, WB
影响因子：
2.4
作者：
M. Aldemir;E. Okulu;S. Neşelioğlu;O. Erel;K. Ener;Ö. Kayıgil
通讯作者：
M. Aldemir;E. Okulu;S. Neşelioğlu;O. Erel;K. Ener;Ö. Kayıgil
影响因子：
7.6
作者：
Whalley, Heather C.;Papmeyer, Martina;McIntosh, Andrew M.
通讯作者：
McIntosh, Andrew M.
影响因子：
2.4
作者：
Aziz, M. T. Abdel;Rezq, A. M.;Yehia, H. M.
通讯作者：
Yehia, H. M.
影响因子：
2.7
作者：
Jiang, Xi;Luo, Yong;Cao, Zhigang
通讯作者：
Cao, Zhigang