A maximum likelihood QTL analysis reveals common genome regions controlling resistance to Salmonella colonization and carrier-state.
A maximum likelihood QTL analysis reveals common genome regions controlling resistance to Salmonella colonization and carrier-state.
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DOI：
10.1186/1471-2164-13-198
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发表时间：
2012-05-21
期刊：
影响因子：
4.4
通讯作者：
Fanny C
中科院分区：
文献类型：
--
作者：
Thanh-Son T;Catherine B;Nigel S;Mark F;Pete K;Elisabeth le BD;Alain V;Philippe V;Fanny C
革兰氏阴性细菌肠道沙门氏菌的血清型肠杆菌和鼠伤寒沙门氏菌是人类食物中毒的重要原因。携带这些细菌的家禽通常没有临床疾病，只有在死后才能检测到。提高商业家禽对带菌者状态的抵抗力可能是通过减少这些细菌在家禽群中的传播来提高食品安全的一种方法。先前的研究在同一白色来航牛近交系中鉴定了对携带状态的抗性和对沙门氏菌定植的抗性的QTL。迄今为止，尚未发现两种抗性共有的QTL。所有这些分析均使用QTLExpress软件的F2近交或回交选项基于线性回归进行。本研究采用最大似然法结合QTLMap软件进行QTL分析，以检验QTL分析方法在QTL检测中的效果。我们分析了与之前研究中使用的相同的表型和基因型数据，这些数据是从378只动物中收集的，使用480个全基因组SNP标记进行基因分型。为了丰富这些数据，我们添加了位于控制定植抗性的QTL内的11个SNP标记，并寻找与QTL共定位的潜在候选基因。在我们的例子中，QTL分析方法对QTL检测有重要影响。我们能够确定新的基因组区域控制抗性载体状态，特别是通过测试存在两个分离的QTL。但一些先前鉴定的QTL没有得到证实。有趣的是，在第2和第3号染色体上检测到两个QTL，靠近控制定植抗性的主要QTL的位置，以及在其他独立研究中确定的参与免疫反应的候选基因。由于检测到的QTL缺乏稳定性，我们建议进一步研究的感兴趣区域是那些在几项独立研究中鉴定的区域，这是染色体2和3上的QTL区域的情况，涉及对沙门氏菌定植和载体状态的抗性。这些观察结果提供了控制S的共同基因的证据。鼠伤寒定殖和S.鸡肠道菌携带者状态。
The serovars Enteritidis and Typhimurium of the Gram-negative bacterium Salmonella enterica are significant causes of human food poisoning. Fowl carrying these bacteria often show no clinical disease, with detection only established post-mortem. Increased resistance to the carrier state in commercial poultry could be a way to improve food safety by reducing the spread of these bacteria in poultry flocks. Previous studies identified QTLs for both resistance to carrier state and resistance to Salmonella colonization in the same White Leghorn inbred lines. Until now, none of the QTLs identified was common to the two types of resistance. All these analyses were performed using the F2 inbred or backcross option of the QTLExpress software based on linear regression. In the present study, QTL analysis was achieved using Maximum Likelihood with QTLMap software, in order to test the effect of the QTL analysis method on QTL detection. We analyzed the same phenotypic and genotypic data as those used in previous studies, which were collected on 378 animals genotyped with 480 genome-wide SNP markers. To enrich these data, we added eleven SNP markers located within QTLs controlling resistance to colonization and we looked for potential candidate genes co-localizing with QTLs. In our case the QTL analysis method had an important impact on QTL detection. We were able to identify new genomic regions controlling resistance to carrier-state, in particular by testing the existence of two segregating QTLs. But some of the previously identified QTLs were not confirmed. Interestingly, two QTLs were detected on chromosomes 2 and 3, close to the locations of the major QTLs controlling resistance to colonization and to candidate genes involved in the immune response identified in other, independent studies. Due to the lack of stability of the QTLs detected, we suggest that interesting regions for further studies are those that were identified in several independent studies, which is the case of the QTL regions on chromosomes 2 and 3, involved in resistance to both Salmonella colonization and carrier state. These observations provide evidence of common genes controlling S. Typhimurium colonization and S. Enteritidis carrier-state in chickens.
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影响因子：
3.1
作者：
Calenge, Fanny;Vignal, Alain;Beaumont, Catherine
通讯作者：
Beaumont, Catherine
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影响因子：
2.4
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影响因子：
2.5
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通讯作者：
MALO, D