Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study.
Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study.
复制标题
乌拉圭牧场奶牛群新生小牛腹泻和死亡的原因：一项农场配对病例对照研究
DOI：
10.1007/s42770-021-00440-3
复制
发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Giannitti F
中科院分区：
文献类型：
--
作者：
Caffarena RD;Casaux ML;Schild CO;Fraga M;Castells M;Colina R;Maya L;Corbellini LG;Riet-Correa F;Giannitti F
新生小牛腹泻（NCD）和死亡率给乳制品行业造成重大损失。乌拉圭的断奶前奶牛犊牛死亡率风险很高（15.2%）;然而，这些损失的原因在很大程度上是未知的。本研究旨在评估各种病原体是否与乌拉圭奶牛的NCD和死亡相关，以及这些感染、腹泻或死亡是否与被动免疫转移（FTPI）失败相关。对来自27个农场的1至30日龄的当代断奶（n = 264）和非断奶（n = 271）小牛进行采样。通过抗原捕获ELISA分析粪便中的隐孢子虫属，轮状病毒、牛冠状病毒和大肠杆菌F5+，牛星状病毒（BoAstV）的RT-PCR，以及肠道沙门氏菌的细菌培养物。通过RT-PCR或抗原捕获ELISA分析血液/血清中的牛病毒性腹泻病毒（BVDV）。用折射法测定95头≤ 8日龄犊牛血清中总蛋白（STP）含量，作为FTPI的指标。记录取样犊牛是否在断奶前死亡。在65.4%的小牛中检测到至少一种病原体，并且该百分比在腹泻小牛（83.7%）中显著高于非腹泻小牛（47.6%）。与其他病原体不同，隐孢子虫属（Cryptosporidium spp.）和轮状病毒与NCD相关。腹泻小牛、感染任何病原体的小牛和感染轮状病毒的小牛的STP浓度显著较低。腹泻小牛在断奶前死亡的几率高于非腹泻小牛。感染沙门氏菌的腹泻小牛。肠道感染者的死亡风险增加。控制非传染性疾病、沙门氏菌病、隐孢子虫病和轮状病毒感染，以及改善初乳管理实践，将有助于降低乌拉圭奶牛场的小牛发病率。在线版本包含补充材料，可通过10.1007/s42770-021-00440-3获得。
Neonatal calf diarrhea (NCD) and mortality cause significant losses to the dairy industry. The preweaning dairy calf mortality risk in Uruguay is high (15.2%); however, causes for these losses are largely unknown. This study aimed to assess whether various pathogens were associated with NCD and death in Uruguayan dairy calves and whether these infections, diarrhea, or deaths were associated with the failure of transfer of passive immunity (FTPI). Contemporary diarrheic (n = 264,) and non-diarrheic (n = 271) 1- to 30-day-old calves from 27 farms were sampled. Feces were analyzed by antigen-capture ELISA for Cryptosporidium spp., rotavirus, bovine coronavirus, and Escherichia coli F5+, RT-PCR for bovine astrovirus (BoAstV), and bacterial cultures for Salmonella enterica. Blood/serum was analyzed by RT-PCR or antigen-capture ELISA for bovine viral diarrhea virus (BVDV). Serum of ≤ 8-day-old calves (n = 95) was assessed by refractometry to determine the concention of serum total proteins (STP) as an indicator of FTPI. Whether the sampled calves died before weaning was recorded. At least one pathogen was detected in 65.4% of the calves, and this percentage was significantly higher in diarrheic (83.7%) versus non-diarrheic (47.6%) calves. Unlike the other pathogens, Cryptosporidium spp. and rotavirus were associated with NCD. Diarrheic calves, calves infected with any of the pathogens, and calves infected with rotavirus had significantly lower concentrations of STP. Diarrheic calves had higher chances of dying before weaning than non-diarrheic calves. Diarrheic calves infected with S. enterica were at increased risk of mortality. Controlling NCD, salmonellosis, cryptosporidiosis, and rotavirus infections, and improving colostrum management practices would help to reduce calf morbi-mortality in dairy farms in Uruguay. The online version contains supplementary material available at 10.1007/s42770-021-00440-3.
登录
查看更多内容
影响因子：
4.7
作者：
Castells, Matias;Bertoni, Estefany;Colina, Rodney
通讯作者：
Colina, Rodney
影响因子：
3.3
作者：
Cho YI;Han JI;Wang C;Cooper V;Schwartz K;Engelken T;Yoon KJ
通讯作者：
Yoon KJ
影响因子：
2.2
作者：
Cuttance, E. L.;Mason, W. A.;Phyn, C. V. C.
通讯作者：
Phyn, C. V. C.
影响因子：
1.1
作者：
Izzo, M. M.;Kirkland, P. D.;House, J. K.
通讯作者：
House, J. K.
影响因子：
2.2
作者：
da Silva Silveira, Caroline;Maya, Leticia;Giannitti, Federico
通讯作者：
Giannitti, Federico