Overexpression of the transcription factor NF-YC9 confers abscisic acid hypersensitivity in Arabidopsis.
Overexpression of the transcription factor NF-YC9 confers abscisic acid hypersensitivity in Arabidopsis.
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转录因子 NF-YC9 的过度表达导致拟南芥脱落酸过敏
DOI：
10.1007/s11103-017-0661-1
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发表时间：
2017-11
影响因子：
5.1
通讯作者：
Zhang DP
中科院分区：
文献类型：
--
作者：
Bi C;Ma Y;Wang XF;Zhang DP
核因子Y(NF-Y)家族蛋白参与植物的许多发育过程和对环境信号的响应，但它们是否以及如何调控植物激素脱落酸(ABA)信号转导尚需进一步研究。在本研究中，我们发现，在幼苗早期生长和气孔反应中，过表达的NF-YC9基因赋予ABA高敏感性，而在这些过程中，NF-YC9的下调并不影响ABA的反应。我们还发现，在幼苗生长的早期，过表达的NF-YC9基因具有盐和渗透敏感性，这可能与ABA的敏感性直接相关。此外，我们观察到，NF-YC9与ABA反应的bZIP转录因子ABA-INSENSITIVE5(ABI5)在物理上相互作用，并促进ABI5结合和激活靶基因EM6的启动子的功能。此外，核因子-YC9上调ABI5基因的表达以响应ABA。这些发现表明，NF-YC9可能作为一个正向调节因子参与ABA信号转导，并可能与其他NF-YC成员一起冗余地发挥作用，并支持了NF-YC9通过靶向和辅助ABI5等ABA反应转录因子来介导ABA信号转导的模型。
Nuclear factor Y (NF-Y) family proteins are involved in many developmental processes and responses to environmental cues in plants, but whether and how they regulate phytohormone abscisic acid (ABA) signaling need further studies. In the present study, we showed that over-expression of the NF-YC9 gene confers ABA hypersensitivity in both the early seedling growth and stomatal response, while down-regulation of NF-YC9 does not affect ABA response in these processes. We also showed that over-expression of the NF-YC9 gene confers salt and osmotic hypersensitivity in early seedling growth, which is likely to be directly associated with the ABA hypersensitivity. Further, we observed that NF-YC9 physically interacts with the ABA-responsive bZIP transcription factor ABA-INSENSITIVE5 (ABI5), and facilitates the function of ABI5 to bind and activate the promoter of a target gene EM6. Additionally, NF-YC9 up-regulates expression of the ABI5 gene in response to ABA. These findings show that NF-YC9 may be involved in ABA signaling as a positive regulator and likely functions redundantly together with other NF-YC members, and support the model that the NF-YC9 mediates ABA signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5.
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DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
5.1
作者：
Jiang, Shang-Chuan;Mei, Chao;Liang, Shan;Yu, Yong-Tao;Lu, Kai;Wu, Zhen;Wang, Xiao-Fang;Zhang, Da-Peng
通讯作者：
Zhang, Da-Peng
影响因子：
3.7
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
影响因子：
3.7
作者：
Kumimoto RW;Siriwardana CL;Gayler KK;Risinger JR;Siefers N;Holt BF 3rd
通讯作者：
Holt BF 3rd
DOI：
10.1199/tab.0114
发表时间：
2008-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Kwak, June M;Maser, Pascal;Schroeder, Julian I
通讯作者：
Schroeder, Julian I