Differential expression of microRNAs and other small RNAs in barley between water and drought conditions.
Differential expression of microRNAs and other small RNAs in barley between water and drought conditions.
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DOI：
10.1111/pbi.12220
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发表时间：
2015-01
影响因子：
13.8
通讯作者：
Shi BJ
中科院分区：
文献类型：
--
作者：
Hackenberg M;Gustafson P;Langridge P;Shi BJ
干旱是作物生产的主要限制因素，而microRNAs（miRNAs）在植物耐旱性中起着重要作用。对在水和干旱条件下生长的大麦中的miRNAs和其他类型的小RNA（sRNAs）的分析表明，干旱选择性地调节miRNAs和其他类型的sRNAs的表达。低表达的miRNAs和所有重复序列相关的siRNAs（rasiRNAs）在干旱条件下倾向于下调，而tRNA衍生的sRNAs（tsRNAs）在干旱条件下倾向于上调。反义sRNAs（推定的siRNAs）在干旱条件下没有这种趋势。在抗旱转基因大麦中，DREB转录因子的低表达也被下调。相比之下，在干旱处理的和转基因植物之间，tsRNA、rasiRNA和其他类型的sRNA并不一致地表达。通过北方杂交和实时定量PCR（qRT-PCR）进一步证实miRNAs和siRNAs的差异表达。预测干旱调节的miRNA和siRNA的靶点，通过degradome文库鉴定并通过qRT-PCR确认。它们的功能多种多样，但大多数都参与转录调控。我们的数据提供了对miRNAs和其他sRNAs的表达谱的深入了解，以及它们在干旱下的关系，从而帮助理解miRNAs和sRNAs如何响应谷类作物的干旱胁迫。
Drought is a major constraint to crop production, and microRNAs (miRNAs) play an important role in plant drought tolerance. Analysis of miRNAs and other classes of small RNAs (sRNAs) in barley grown under water and drought conditions reveals that drought selectively regulates expression of miRNAs and other classes of sRNAs. Low-expressed miRNAs and all repeat-associated siRNAs (rasiRNAs) tended towards down-regulation, while tRNA-derived sRNAs (tsRNAs) had the tendency to be up-regulated, under drought. Antisense sRNAs (putative siRNAs) did not have such a tendency under drought. In drought-tolerant transgenic barley overexpressing DREB transcription factor, most of the low-expressed miRNAs were also down-regulated. In contrast, tsRNAs, rasiRNAs and other classes of sRNAs were not consistently expressed between the drought-treated and transgenic plants. The differential expression of miRNAs and siRNAs was further confirmed by Northern hybridization and quantitative real-time PCR (qRT-PCR). Targets of the drought-regulated miRNAs and siRNAs were predicted, identified by degradome libraries and confirmed by qRT-PCR. Their functions are diverse, but most are involved in transcriptional regulation. Our data provide insight into the expression profiles of miRNAs and other sRNAs, and their relationship under drought, thereby helping understand how miRNAs and sRNAs respond to drought stress in cereal crops.
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