Evidence for and against deformed wing virus spillover from honey bees to bumble bees: a reverse genetic analysis.
Evidence for and against deformed wing virus spillover from honey bees to bumble bees: a reverse genetic analysis.
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DOI：
10.1038/s41598-020-73809-3
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发表时间：
2020-10-08
影响因子：
4.6
通讯作者：
Evans DJ
中科院分区：
文献类型：
--
作者：
Gusachenko ON;Woodford L;Balbirnie-Cumming K;Ryabov EV;Evans DJ
变形翅病毒是欧洲蜜蜂的一种持久性病原，是越冬蜂群损失的主要原因。黄蜂病毒在蜜蜂中的流行引起了人们对该病毒对其他授粉物种溢出的严重担忧。大黄蜂既是野生和商业饲养的传粉者的主要群体。一些研究已经报道了DWV从蜜蜂到大黄蜂的病原体溢出，但以病毒复制和积累为特征的持续病毒感染的证据尚未得到证明。本文以黄尾熊蜂Bombus terrestris为模型，研究黄蜂病毒在熊蜂体内的感染性和传播性。我们应用反向遗传学方法结合受控的实验室条件来检测和监测DWV感染。使用了一种新的反向遗传学系统，对三种具有代表性的DWV变体，包括DWV的两个主变异体-A型和B型-进行了研究。我们的结果直接证实了大黄蜂体内的DWV复制，但也显示出对某些传播途径感染的显著抵抗力。大黄蜂可能支持DWV复制，但尚不清楚在自然环境条件下如何发生感染。
Deformed wing virus (DWV) is a persistent pathogen of European honey bees and the major contributor to overwintering colony losses. The prevalence of DWV in honey bees has led to significant concerns about spillover of the virus to other pollinating species. Bumble bees are both a major group of wild and commercially-reared pollinators. Several studies have reported pathogen spillover of DWV from honey bees to bumble bees, but evidence of a sustained viral infection characterized by virus replication and accumulation has yet to be demonstrated. Here we investigate the infectivity and transmission of DWV in bumble bees using the buff-tailed bumble bee Bombus terrestris as a model. We apply a reverse genetics approach combined with controlled laboratory conditions to detect and monitor DWV infection. A novel reverse genetics system for three representative DWV variants, including the two master variants of DWV—type A and B—was used. Our results directly confirm DWV replication in bumble bees but also demonstrate striking resistance to infection by certain transmission routes. Bumble bees may support DWV replication but it is not clear how infection could occur under natural environmental conditions.
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