Cellular diversity and gene expression profiles in the male and female brain of Aedes aegypti.
Cellular diversity and gene expression profiles in the male and female brain of Aedes aegypti.
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DOI：
10.1186/s12864-022-08327-9
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发表时间：
2022-02-10
期刊：
影响因子：
4.4
通讯作者：
Franz AWE
中科院分区：
文献类型：
--
作者：
Cui Y;Behura SK;Franz AWE
埃及伊蚊是一种医学上重要的蚊媒，可将包括黄热病、登革热、基孔肯雅病和寨卡病毒在内的虫媒病毒传播给人类。蚊子表现出典型的性二态行为，例如求爱、交配、寻找宿主、吸血和产卵。所有这些行为主要由大脑调节;然而，对蚊子大脑的功能和神经元组成知之甚少。在这项研究中，我们生成了成年男性和女性大脑的Ae的初始图谱。使用基于10 xGenomics的单核RNA测序对埃及伊蚊进行测序。我们在男性和女性大脑中识别出35个脑细胞簇，其中15个被分配到已知的细胞类型。已鉴定的细胞类型包括神经胶质（星形胶质细胞）、Kenyon细胞、（腹侧）投射神经元、单胺能神经元、髓质神经元和近端髓质神经元。此外，雄性和雌性大脑的细胞类型组成进行了比较，显示它们在数量上是不同的，因为35个细胞簇中有17个细胞类型比例显著不同。总体而言，来自每个细胞簇的转录组在男性和女性大脑之间看起来非常相似，因为在这些簇中只有多达25个基因差异表达。性别决定因子Nix在雄性大脑的神经元和神经胶质细胞中高度表达，而doubloxex（dsx）在雄性和雌性大脑的所有神经元和神经胶质细胞簇中表达。蚊子Ae的大脑的初始细胞图谱。已经产生了埃及伊蚊，显示这种吸血昆虫的雄性和雌性大脑的细胞组成彼此显著不同。虽然在我们的单个生物复制品中没有检测到一些罕见的脑细胞类型，但这项研究为进一步开发完整的脑细胞图谱以及更好地了解雄性和雌性蚊子大脑的神经生物学及其性二态行为提供了重要基础。在线版本包含补充材料，可通过10.1186/s12864-022-08327-9获得。
Aedes aegypti is a medically-important mosquito vector that transmits arboviruses including yellow fever, dengue, chikungunya, and Zika viruses to humans. The mosquito exhibits typical sexually dimorphic behaviors such as courtship, mating, host seeking, bloodfeeding, and oviposition. All these behaviors are mainly regulated by the brain; however, little is known about the function and neuron composition of the mosquito brain. In this study, we generated an initial atlas of the adult male and female brain of Ae. aegypti using 10xGenomics based single-nucleus RNA sequencing. We identified 35 brain cell clusters in male and female brains, and 15 of those clusters were assigned to known cell types. Identified cell types include glia (astrocytes), Kenyon cells, (ventral) projection neurons, monoaminergic neurons, medulla neurons, and proximal medulla neurons. In addition, the cell type compositions of male and female brains were compared to each other showing that they were quantitatively distinct, as 17 out of 35 cell clusters varied significantly in their cell type proportions. Overall, the transcriptomes from each cell cluster looked very similar between the male and female brain as only up to 25 genes were differentially expressed in these clusters. The sex determination factor Nix was highly expressed in neurons and glia of the male brain, whereas doublesex (dsx) was expressed in all neuron and glia cell clusters of the male and female brain. An initial cell atlas of the brain of the mosquito Ae. aegypti has been generated showing that the cellular compositions of the male and female brains of this hematophagous insect differ significantly from each other. Although some of the rare brain cell types have not been detected in our single biological replicate, this study provides an important basis for the further development of a complete brain cell atlas as well as a better understanding of the neurobiology of the brains of male and female mosquitoes and their sexually dimorphic behaviors. The online version contains supplementary material available at 10.1186/s12864-022-08327-9.
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影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
中肠细胞的异质性及其对蚊子艾德斯埃及摄入血餐的差异反应。
影响因子：
3.8
作者：
Cui Y;Franz AWE
通讯作者：
Franz AWE
影响因子：
8.8
作者：
Jenett, Arnim;Rubin, Gerald M.;Zugates, Christopher T.
通讯作者：
Zugates, Christopher T.
影响因子：
16.2
作者：
Jove, Veronica;Gong, Zhongyan;Hol, Felix J. H.;Zhao, Zhilei;Sorrells, Trevor R.;Carroll, Thomas S.;Prakash, Manu;McBride, Carolyn S.;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z