Controlled etching of gold nanorods by the Au(III)-CTAB complex, and its application to semi-quantitative visual determination of organophosphorus pesticides
Controlled etching of gold nanorods by the Au(III)-CTAB complex, and its application to semi-quantitative visual determination of organophosphorus pesticides
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Au(III)-CTAB配合物对金纳米棒的控制蚀刻及其在有机磷农药半定量目视测定中的应用
DOI：
10.1007/s00604-017-2468-9
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发表时间：
2017-08
影响因子：
5.7
通讯作者：
Wang Jiamian
中科院分区：
文献类型：
--
作者：
Wu Shuo;Li D;an;Gao Zhanming;Wang Jiamian
本文介绍了一种目视半定量检测有机磷农药的比色法。它是基于金纳米棒（AuNR）的纵横比依赖的吸收，这是由于局部表面等离子体共振。纵横比可以通过与十六烷基三甲基溴化铵（CTAB）形成的Au（III）络合物来定制，所述络合物受基于乙酰胆碱酯酶（AChE）的水解的影响。OP充当该反应的抑制剂。在没有OP的情况下，酶水解的产物（硫代胆碱）消耗几乎所有的Au（III）物质。在这种情况下，残留的Au（III）-CTAB络合物的量少，并且不会发生AuNR的蚀刻。然而，在OP的存在下，AChE的活性被抑制。因此，仅产生少量（或不产生）硫代胆碱，并且不消耗Au（III）。Au（III）能够蚀刻AuNR并改变它们的纵横比。这导致颜色从褐色变为灰色、青色、绿色、蓝色、紫色、红色和无色，这可以用裸眼容易地观察到。将AuNR掺入纤维素纸中以获得用于视觉检测OP的纸条。在最佳条件下，这两种方法（AuNR在水溶液中和纸上）允许各种OP被确定。方法的检出限为1.2ppb，线性范围为0.01 ~ 1.84ppm。将该方法应用于白菜洗涤液和海水样品的分析，结果表明，该方法具有良好的分离度和准确度，可用于农产品和食品中有机磷农药的现场检测。
图形摘要基于金纳米棒蚀刻的有机磷农药视觉检测半定量方法示意图。可以观察到从褐色到灰色、绿色、蓝色、紫色、红色和无色的颜色变化，并且与农药的浓度相关。
AbstractThe authors describe a semi-quantitative colorimetric method for visual detection of organophosphorus pesticides (OPs). It is based on the aspect ratio dependent absorbance of gold nanorods (AuNRs) which is due to the localized surface plasmon resonance. The aspect ratio can be tailored by the Au(III) complex formed with cetyltrimethylammonium bromide (CTAB) which is affected by acetylcholinesterase (AChE) based hydrolysis. OPs act as inhibitors of this reaction. In the absence of OPs, the product (thiocholine) of enzymatic hydrolysis consumes almost all the Au(III) species. In this case, the amount of residual Au(III)-CTAB complexes is small, and etching of the AuNRs does not occur. In the presence of OPs, however, the activity of AChE is inhibited. Hence, only small quantities of (or no) thiocholine is produced, and Au(III) is not consumed. Au(III) is capable of etching AuNRs and to change their aspect ratio. This leads to a color change from brownish to gray, cyan, green, blue, purple, red, and colorless that can be easily observed with bare eyes. The AuNRs were incorporated into cellulose paper to obtain a paper stripe for visual detection of OPs. Under optimal conditions, both methods (AuNRs in aqueous solutions and on paper) allowed various OPs to be determined. Applied to parathion, the method had a detection limit as low as 1.2 ppb and a linear range from 0.01 to 1.84 ppm. It was applied to the analysis of cabbage washing solutions and sea water samples, and acceptable accuracy and good resolution were found. In our preconception, the method represents a valuable tool for on-site detection of OPs in agriculture products and food.
Graphical abstractSchematic of a semi-quantitative method for the visual detection of organophosphate pesticides based on etching of gold nanorods. A color change from brownish to gray, green, blue, purple, red, and colorless can be observed and correlated to the concentration of pesticides.
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影响因子：
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