Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
Relationships between TGFbeta proteins and oxygen concentrations inside the first trimester human gestational sac.
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DOI：
10.1371/journal.pone.0002302
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发表时间：
2008-06-04
期刊：
影响因子：
3.7
通讯作者：
Jauniaux, Eric
中科院分区：
文献类型：
--
作者：
Muttukrishna, Shanthi;Suri, Sangeeta;Groome, Nigel;Jauniaux, Eric
在妊娠早期，母体循环和妊娠囊组织之间的 O2 梯度调节滋养层的生物学功能。目的是评估胎盘氧分压 (PaO2) 是否调节妊娠早期妊娠囊内特定胎盘蛋白的体内合成。在 37 例妊娠 6-12 周的正常妊娠中获得了外周静脉血、胎盘床血液 (PB)、体腔液 (CF) 和胎盘组织的匹配样本。使用 IRMA 血气监测仪测量 PB 和 CF 中的 PaO2。测量所有样品中的抑制素 A、激活素 A、sEng、PlGF、sFlt-1 和游离 VEGF 浓度。在胎盘提取物中测量 HSP 70。方差分析显示妊娠 10 周后 PB PaO2 增加约 60% (P = 0.02)。两组之间不配对的学生 T 检验（6-9 周 vs 9-12 周）显示 MS 激活素 A (P = 0.001)、CF 激活素 A (P<0.001)、MS P1GF (P = 0.001)、CF PlGF (P<0.001)、MS sFLT-1 显着增加(P = 0.03)、CF sFLT-1 (P = 0.01)、胎盘提取物中的 HSP 70 (P = 0.04) 以及 PB 抑制素 A 水平显着降低 (P<0.001) 和 PB sFLT-1 (P = 0.02)。多重相关分析显示PB抑制素A水平与妊娠（r = −0.45，P<0.05）和PB PaO2（r = −0.5，P = 0.008）以及sFLT-1和PB PaO2之间呈显着负相关（P = 0.03）。胎盘提取物中PlGF、sEng和VEGF水平呈正相关（P<0.01）。我们的结果表明体内早期宫内 PaO2 与抑制素 A 和 sFLT-1 浓度之间存在直接关系，证实了我们的假设，即特定胎盘蛋白受宫内 O2 张力调节。
In early pregnancy, the O2 gradient between the maternal circulation and the gestational sac tissues modulates trophoblast biological functions. The aim was to evaluate if placental partial pressure of oxygen (PaO2) modulates in vivo synthesis of specific placental proteins inside the first trimester gestational sac. Matched samples of peripheral venous blood, blood from the placental bed (PB), coelomic fluid (CF) and placental tissue were obtained in 37 normal pregnancies at 6–12 weeks gestation. PaO2 was measured in PB and CF using an IRMA blood gas monitor. Inhibin A, activin A, sEng, PlGF, sFlt-1 and free VEGF concentrations were measured in all samples. HSP 70 was measured in placental extracts. ANOVA showed ∼60% increase in PB PaO2 (P = 0.02) between after 10 weeks gestation. Unpaired Student's T-test between two groups (6–9 weeks vs 9–12 weeks) shows a significant increase in MS Activin A (P = 0.001), CF activin A (P<0.001), MS P1GF (P = 0.001), CF PlGF (P<0.001), MS sFLT-1 (P = 0.03), CF sFLT-1 (P = 0.01), HSP 70 in placental extracts (P = 0.04) and a significant decrease in PB inhibin A levels (P<0.001) and PB sFLT-1 (P = 0.02) . Multiple correlation analysis showed a significant negative correlation between PB inhibin A levels and gestation (r = −0.45, P<0.05) and PB PaO2 (r = −0.5, P = 0.008) and also between sFLT-1 and PB PaO2 (P = 0.03). There was a positive correlation (P<0.01) between PlGF, sEng and VEGF levels in the placental extracts. Our results indicate a direct relationship in the early intrauterine PaO2 in vivo and inhibin A and sFLT-1 concentrations confirming our hypothesis that specific placental proteins are regulated by intrauterine O2 tension.
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影响因子：
3.6
作者：
Clark, DE;Smith, SK;Charnock-Jones, DS
通讯作者：
Charnock-Jones, DS
影响因子：
2.7
作者：
Muttukrishna, S
通讯作者：
Muttukrishna, S
影响因子：
5.8
作者：
Jauniaux, E;Hempstock, J;Burton, GJ
通讯作者：
Burton, GJ
影响因子：
56.9
作者：
Genbacev, O;Zhou, Y;Fisher, SJ
通讯作者：
Fisher, SJ
DOI：
10.1111/j.1471-0528.1986.tb07830.x
发表时间：
1986-10-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
KHONG, TY;DEWOLF, F;BROSENS, I
通讯作者：
BROSENS, I