Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations.
Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations.
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DOI：
10.1038/srep34843
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发表时间：
2016-10-06
影响因子：
4.6
通讯作者：
de Leeuw DM
中科院分区：
文献类型：
--
作者：
Rocha PR;Schlett P;Kintzel U;Mailänder V;Vandamme LK;Zeck G;Gomes HL;Biscarini F;de Leeuw DM
微电极阵列（MEA）记录粘附在电极上的细胞的细胞外局部场电位。缺点是信噪比有限。最先进的背景噪声水平约为 10 μVpp。此外，在 MEA 中，低频事件被滤除。在这里，我们通过阻抗谱和噪声测量来定量分析金电极/电解质界面。等效电路是电荷转移电阻与描述双层电容的恒定相位元件并联，并与扩散电阻串联。该等效电路产生麦克斯韦-瓦格纳弛豫频率，其值根据电极面积和 KCl 电解质水溶液的摩尔浓度确定。电化学电压和电流噪声作为电极面积和频率的函数进行测量，并且明确地从测量的阻抗得出。通过使用大面积电极，本底噪声可低至 0.3 μVpp。 C6 神经胶质瘤细胞群的细胞外检测证明了由此产生的高灵敏度。它们的微小电活动可以在低于约 10 Hz 的频率下清晰地检测到，这表明该方法可用于监测细胞群中缓慢的协作生物信号。
Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 μVpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.
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DOI：
10.1523/jneurosci.2041-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Manning JR;Jacobs J;Fried I;Kahana MJ
通讯作者：
Kahana MJ
影响因子：
4.3
作者：
Obien ME;Deligkaris K;Bullmann T;Bakkum DJ;Frey U
通讯作者：
Frey U
影响因子：
--
作者：
Nyquist, H
通讯作者：
Nyquist, H
影响因子：
2.5
作者：
Hutzler, M.;Lambacher, A.;Fromherz, P.
通讯作者：
Fromherz, P.
影响因子：
3.9
作者：
Hirschorn, Bryan;Orazem, Mark E.;Musiani, Marco
通讯作者：
Musiani, Marco