Human adipose ECM alleviates radiation-induced skin fibrosis via endothelial cell-mediated M2 macrophage polarization.
Human adipose ECM alleviates radiation-induced skin fibrosis via endothelial cell-mediated M2 macrophage polarization.
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人脂肪ECM通过内皮细胞介导的M2巨噬细胞极化抑制辐射诱导的皮肤纤维化
DOI：
10.1016/j.isci.2023.107660
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发表时间：
2023-09-15
期刊：
影响因子：
5.8
通讯作者：
Ejaz, Asim
中科院分区：
文献类型：
--
作者：
Chinnapaka, Somaiah;Yang, Katherine S.;Surucu, Yusuf;Bengur, Fuat B.;Arellano, Jose A.;Tirmizi, Zayaan;Malekzadeh, Hamid;Epperly, Michael W.;Hou, Wen;Greenberger, Joel S.;Rubin, J. Peter;Ejaz, Asim
放射治疗可导致晚期放射性皮肤纤维化（RISF），引起运动受限、疼痛和器官功能障碍。本研究评估了脂肪来源的细胞外基质（Ad-ECM）作为RISF的缓解剂。经辐照的雌性C57 BL/6 J小鼠出现纤维化，其通过单次局部Ad-ECM注射减轻，改善肢体运动并减少上皮厚度和胶原沉积。Ad-ECM治疗导致促炎基因和纤维化基因表达减少，抗炎细胞因子上调，促进M2巨噬细胞极化。照射的人成纤维细胞与Ad-ECM的共培养下调纤维化基因表达并增强骨髓细胞迁移。Ad-ECM治疗还增加了内皮细胞中白细胞介素（IL）-4，IL-5和IL-15的表达，刺激M2巨噬细胞极化并减轻RISF。预防性使用Ad-ECM显示出缓解效果。这项研究表明Ad-ECM在治疗慢性纤维化方面的潜力。Ad-ECM可有效缓解RISF M2巨噬细胞极化在缓解中起着至关重要的作用Ad-ECM为RISF提供预防性缓解潜力细胞生物学;皮肤病学;纤维化;免疫学;干细胞研究
Radiation therapy can lead to late radiation-induced skin fibrosis (RISF), causing movement restriction, pain, and organ dysfunction. This study evaluated adipose-derived extracellular matrix (Ad-ECM) as a mitigator of RISF. Female C57BL/6J mice that were irradiated developed fibrosis, which was mitigated by a single local Ad-ECM injection, improving limb movement and reducing epithelium thickness and collagen deposition. Ad-ECM treatment resulted in decreased expression of pro-inflammatory and fibrotic genes, and upregulation of anti-inflammatory cytokines, promoting M2 macrophage polarization. Co-culture of irradiated human fibroblasts with Ad-ECM down-modulated fibrotic gene expression and enhanced bone marrow cell migration. Ad-ECM treatment also increased interleukin (IL)-4, IL-5, and IL-15 expression in endothelial cells, stimulating M2 macrophage polarization and alleviating RISF. Prophylactic use of Ad-ECM showed effectiveness in mitigation. This study suggests Ad-ECM’s potential in treating chronic-stage fibrosis. Ad-ECM can effectively mitigate RISF M2 macrophage polarization plays a crucial role in mitigation Ad-ECM offers prophylactic mitigation potential to RISF Cell biology; Dermatology; Fibrosis; Immunology; Stem cells research
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影响因子：
3.6
作者：
Garza RM;Paik KJ;Chung MT;Duscher D;Gurtner GC;Longaker MT;Wan DC
通讯作者：
Wan DC
影响因子：
7.8
作者：
Huleihel L;Dziki JL;Bartolacci JG;Rausch T;Scarritt ME;Cramer MC;Vorobyov T;LoPresti ST;Swineheart IT;White LJ;Brown BN;Badylak SF
通讯作者：
Badylak SF
影响因子：
1
作者：
Akita, S.;Yoshimoto, H.;Yamashita, S.
通讯作者：
Yamashita, S.
辐照组织中细胞因子和生长因子表达的长期改变以及与组织学严重程度评分的关系。
影响因子：
3.7
作者：
Gallet P;Phulpin B;Merlin JL;Leroux A;Bravetti P;Mecellem H;Tran N;Dolivet G
通讯作者：
Dolivet G
影响因子：
14
作者：
Brown, Bryan N.;Valentin, Jolene E.;Stewart-Akers, Ann M.;McCabe, George P.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.