Brevinin-2GHk, a Peptide Derived from the Skin of Fejervarya limnocharis, Inhibits Zika Virus Infection by Disrupting Viral Integrity.
Brevinin-2GHk, a Peptide Derived from the Skin of Fejervarya limnocharis, Inhibits Zika Virus Infection by Disrupting Viral Integrity.
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Brevinin-2GHk 是一种从 Fejervarya limnocharis 皮肤中提取的肽，通过破坏病毒完整性来抑制寨卡病毒感染
DOI：
10.3390/v13122382
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发表时间：
2021-11-28
期刊：
影响因子：
--
通讯作者：
Zou M
中科院分区：
文献类型：
--
作者：
Xiong W;Li J;Feng Y;Chai J;Wu J;Hu Y;Tian M;Lu W;Xu X;Zou M
自2015 - 2016年寨卡病毒（ZIKV）大流行再次发生以来已经过去了几年。然而，仍然缺乏针对ZIKV的经证实的保护性疫苗或有效药物。属于抗微生物肽brevinin-2家族的肽brevinin-2GHk（BR2GK）已被报道仅显示出弱的抗菌活性，并且其抗病毒作用尚未被研究。因此，我们分析了BR2GK对ZIKV感染的作用。BR2GK在ZIKV感染的早期和中期显示出显著的抑制活性，具有可忽略的细胞毒性。此外，BR2GK被认为与ZIKV E蛋白结合并破坏包膜的完整性，从而直接灭活ZIKV。此外，BR2GK还可以穿透细胞膜，这可能有助于抑制ZIKV感染的中期。BR2GK以3.408 ± 0.738 μ M的IC50阻断ZIKV E蛋白表达。总之，发现BR2GK是多功能候选物和用于进一步开发抗ZIKV药物的潜在先导化合物。
Several years have passed since the Zika virus (ZIKV) pandemic reoccurred in 2015–2016. However, there is still a lack of proved protective vaccines or effective drugs against ZIKV. The peptide brevinin-2GHk (BR2GK), pertaining to the brevinin-2 family of antimicrobial peptides, has been reported to exhibit only weak antibacterial activity, and its antiviral effects have not been investigated. Thus, we analyzed the effect of BR2GK on ZIKV infection. BR2GK showed significant inhibitory activity in the early and middle stages of ZIKV infection, with negligible cytotoxicity. Furthermore, BR2GK was suggested to bind with ZIKV E protein and disrupt the integrity of the envelope, thus directly inactivating ZIKV. In addition, BR2GK can also penetrate the cell membrane, which may contribute to inhibition of the middle stage of ZIKV infection. BR2GK blocked ZIKV E protein expression with an IC50 of 3.408 ± 0.738 μΜ. In summary, BR2GK was found to be a multi-functional candidate and a potential lead compound for further development of anti-ZIKV drugs.
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影响因子：
4.9
作者：
Marcocci ME;Amatore D;Villa S;Casciaro B;Aimola P;Franci G;Grieco P;Galdiero M;Palamara AT;Mangoni ML;Nencioni L
通讯作者：
Nencioni L
影响因子：
7.6
作者：
Kamiyama N;Soma R;Hidano S;Watanabe K;Umekita H;Fukuda C;Noguchi K;Gendo Y;Ozaki T;Sonoda A;Sachi N;Runtuwene LR;Miura Y;Matsubara E;Tajima S;Takasaki T;Eshita Y;Kobayashi T
通讯作者：
Kobayashi T
影响因子：
7.5
作者：
Nguyen, Tienthanh;Chen, Xin;Xu, Xueqing
通讯作者：
Xu, Xueqing
影响因子：
3.7
作者：
Lok SM;Costin JM;Hrobowski YM;Hoffmann AR;Rowe DK;Kukkaro P;Holdaway H;Chipman P;Fontaine KA;Holbrook MR;Garry RF;Kostyuchenko V;Wimley WC;Isern S;Rossmann MG;Michael SF
通讯作者：
Michael SF
DOI：
10.3390/antibiotics9100661
发表时间：
2020-09-30
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Rollins-Smith LA;Smith PB;Ledeczi AM;Rowe JM;Reinert LK
通讯作者：
Reinert LK