Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping.
Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping.
复制标题
DOI：
10.1002/jbm.a.34181
复制
发表时间：
2012-09
影响因子：
4.9
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Roy, Mangal;Bose, Susmita
骨替代材料需要支持骨重建过程，该过程包括骨吸收和成骨细胞合成。破骨细胞是由造血单核细胞分化而成的骨吸收细胞。在本研究中，我们评估了在β-磷酸三钙（β-TCP）中掺杂1.0wt%的锶（Sr）和1.0wt%的镁（Mg）对单核细胞向破骨细胞样细胞分化及其吸收活性的影响。采用破骨细胞前体RAW 264.7细胞，添加核因子κβ受体激活剂配体（RANKL），研究体外破骨细胞样细胞的形成、粘附和吸收。在第8天，在纯的和Sr掺杂的β-TCP样品上观察到破骨细胞样细胞形成，这在Mg掺杂的β-TCP样品上不存在，表明由于Mg掺杂导致的初始破骨细胞分化的减少。培养21天后，在所有具有成骨细胞标志物（如肌动蛋白环、多核和存在玻连蛋白受体αvβ3整联蛋白）的样品上，破骨细胞样细胞形成明显。破骨细胞分化后，所有基质均显示破骨细胞样细胞介导的降解，然而，Mg掺杂的β-TCP样品明显受限。我们目前的研究结果表明，基质化学控制破骨细胞的分化和吸收活性，可用于设计TCP为基础的可吸收骨替代品与控制降解性能。
Bone substitute materials are required to support the remodeling process, which consists of osteoclastic resorption and osteoblastic synthesis. Osteoclasts, the bone resorbing cells, generate from differentiation of hemopoietic mononuclear cells. In the present study we have evaluated the effects of 1.0 wt% strontium (Sr) and 1.0 wt% magnesium (Mg) doping in beta-tricalcium phosphate (β-TCP) on the differentiation of mononuclear cells into osteoclast-like cells and its resorptive activity. In vitro osteoclast-like cell formation, adhesion, and resorption were studied using osteoclast precursor RAW 264.7 cell, supplemented with receptor activator of nuclear factor κβ ligand (RANKL). Osteoclast-like cell formation was noticed on pure and Sr doped β-TCP samples at day 8 which was absent on Mg doped β-TCP samples indicating decrease in initial osteoclast differentiation due to Mg doping. After 21 days of culture, osteoclast-like cell formation was evident on all samples with osteoclastic markers such as actin ring, multiple nuclei, and presence of vitronectin receptor αvβ3 integrin. After osteoclast differentiation, all substrates showed osteoclast-like cell mediated degradation, however; significantly restricted for Mg doped β-TCP samples. Our present results indicated substrate chemistry controlled osteoclast differentiation and resorptive activity which can be used in designing TCP based resorbable bone substitutes with controlled degradation properties.
登录
查看更多内容
影响因子：
9.7
作者：
Li, Xia;Ito, Atsuo;Sogo, Yu;Wang, Xiupeng;LeGeros, R. Z.
通讯作者：
LeGeros, R. Z.
DOI：
10.1016/j.msec.2006.12.010
发表时间：
2008-01-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Seeley, Zachary;Bandyopadhyay, Amit;Bose, Susmita
通讯作者：
Bose, Susmita
影响因子：
9.7
作者：
Capuccini, C.;Torricelli, P.;Bigi, A.
通讯作者：
Bigi, A.
影响因子：
9.7
作者：
Detsch, R.;Hagmeyer, D.;Epple, M.
通讯作者：
Epple, M.
影响因子：
56.9
作者：
Salo, J;Lehenkari, P;Vaananen, HK
通讯作者：
Vaananen, HK