Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate.
Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate.
复制标题
DOI：
10.1186/1471-2164-11-543
复制
发表时间：
2010-10-07
期刊：
影响因子：
4.4
通讯作者：
Andersson B
中科院分区：
文献类型：
--
作者：
Jerlström-Hultqvist J;Franzén O;Ankarklev J;Xu F;Nohýnková E;Andersson JO;Svärd SG;Andersson B
肠贾第虫是一种原生动物寄生虫，在许多哺乳动物物种中引起腹泻。为了进一步了解肠贾第鞭毛虫物种之间的遗传多样性，我们对从猪中分离的E组贾第鞭毛虫野生型样本进行了基因组测序和分析。我们鉴定了5012个蛋白质编码基因，与之前测序的WB和GS菌株的基因组相比，其中大多数在微合成和序列一致性方面是保守的。尽管如此，出乎意料的是，在所有染色体中都存在大量的染色体重排和一些较小的结构变化。发现了VSP、NEK激酶和HCMP基因家族的新成员，这可能揭示宿主特异性的可能机制和抗原变异的新途径。研究人员对三种不同的肠贾第虫分离株P15、GS和WB进行了比较基因组学分析，确定了该物种复合体的核心蛋白质组，并鉴定了谱系特异性基因。对贾第鞭毛虫核心蛋白质组多态性的广泛分析揭示了细胞过程中差异的分化率。我们的研究结果表明，尽管贾第鞭毛虫分离株具有保守的核心基因，但在基因含量、基因多态性、结构染色体变异和表面分子谱方面存在显著的基因组差异。该研究改进了贾第鞭毛虫基因组的注释，并使识别功能重要的变异成为可能。
Giardia intestinalis is a protozoan parasite that causes diarrhea in a wide range of mammalian species. To further understand the genetic diversity between the Giardia intestinalis species, we have performed genome sequencing and analysis of a wild-type Giardia intestinalis sample from the assemblage E group, isolated from a pig. We identified 5012 protein coding genes, the majority of which are conserved compared to the previously sequenced genomes of the WB and GS strains in terms of microsynteny and sequence identity. Despite this, there is an unexpectedly large number of chromosomal rearrangements and several smaller structural changes that are present in all chromosomes. Novel members of the VSP, NEK Kinase and HCMP gene families were identified, which may reveal possible mechanisms for host specificity and new avenues for antigenic variation. We used comparative genomics of the three diverse Giardia intestinalis isolates P15, GS and WB to define a core proteome for this species complex and to identify lineage-specific genes. Extensive analyses of polymorphisms in the core proteome of Giardia revealed differential rates of divergence among cellular processes. Our results indicate that despite a well conserved core of genes there is significant genome variation between Giardia isolates, both in terms of gene content, gene polymorphisms, structural chromosomal variations and surface molecule repertoires. This study improves the annotation of the Giardia genomes and enables the identification of functionally important variation.
登录
查看更多内容
影响因子：
3.6
作者：
Goto, R;Panter-Brick, C;Tuladhar, NR
通讯作者：
Tuladhar, NR
影响因子：
2.2
作者：
Ey, PL;Mansouri, M;Mayrhofer, G
通讯作者：
Mayrhofer, G
DOI：
10.1046/j.1440-1746.2000.02089.x
发表时间：
2000-03-01
影响因子：
4.1
作者：
Hellard, ME;Sinclair, MI;Fairley, CK
通讯作者：
Fairley, CK
影响因子：
9.2
作者：
Cooper, Margarethe A.;Adam, Rodney D.;Sterling, Charles R.
通讯作者：
Sterling, Charles R.
影响因子：
4
作者：
Felipe Jimenez-Garcia, Luis;Zavala, Guadalupe;Ortega-Pierres, Guadalupe
通讯作者：
Ortega-Pierres, Guadalupe