Compression Testing and Modeling of Spherical Cells – Comparison of Yeast and Algae

Compression Testing and Modeling of Spherical Cells – Comparison of Yeast and Algae
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球形细胞的压缩测试和建模 - 酵母和藻类的比较

DOI:
10.1002/ceat.201600145
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发表时间:
2017
影响因子:
2.1
通讯作者:
Kampen
Kampen
中科院分区:
工程技术4区
文献类型:
--
作者:
Overbeck;Günther;Steffi;Kampen

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使用单细胞压缩实验对酵母酿酒酵母和微藻小球藻进行机械表征。微机械单元特性可以直接从力位移数据计算出来。由于细胞壁的内在参数无法直接测量,因此细胞壁的力学性能由不同的分析模型确定。正如预期的那样,从细胞模型和细胞壁模型获得的杨氏模量相差两个数量级。尽管酵母和藻类细胞需要类似的力才能破裂,但藻类细胞的破裂变形和破裂能量大约小两倍。基于干生物质,C的细胞破碎的能量。 vulgarisi 比 S 大约高六倍。 酿酒酵母。最后,计算细胞壁的拉伸强度。
The yeastSaccharomyces cerevisiaeand the microalgaChlorella vulgariswere mechanically characterized using single‐cell compression experiments. Micromechanical cell characteristics could be calculated directly from the force displacement data. The mechanical properties of the cell wall were determined with different analytical models because the intrinsic parameters of the cell wall cannot be measured directly. As expected, the Young's moduli obtained from the cellular model and the cell wall model differ by two orders of magnitude. Although yeast and algal cells need similar forces to get disrupted, the bursting deformation and bursting energy of the algal cells are about two times smaller. Based on the dry biomass, the energy for cell breakage ofC. vulgarisis about six times higher than that ofS. cerevisiae. Finally, the tensile strength of the cell wall was calculated.
DOI: 10.1002/ceat.201400632
发表时间: 2016
影响因子: 2.1
作者:
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